miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27234 3' -51.1 NC_005832.1 + 87327 0.66 0.9812
Target:  5'- cCCcAGUGacgggaGUCUCUGUCg---CCGACa -3'
miRNA:   3'- -GGuUCAUg-----CGGAGACGGaaaaGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 71426 0.66 0.97255
Target:  5'- gCAAaaACGCuCUCUGCCUgcuuggguccaCCGACa -3'
miRNA:   3'- gGUUcaUGCG-GAGACGGAaaa--------GGCUG- -5'
27234 3' -51.1 NC_005832.1 + 1367 0.67 0.967612
Target:  5'- -gGAGUGCcCCUCUcGCCguggUCCGGg -3'
miRNA:   3'- ggUUCAUGcGGAGA-CGGaaa-AGGCUg -5'
27234 3' -51.1 NC_005832.1 + 23761 0.67 0.95672
Target:  5'- gCCu-GUACGCCggcCUGCCUUacgCCGcCc -3'
miRNA:   3'- -GGuuCAUGCGGa--GACGGAAaa-GGCuG- -5'
27234 3' -51.1 NC_005832.1 + 14271 0.67 0.952592
Target:  5'- aCCGAGgacACGuuguCCUCUGCCUgggUUUCUGcCa -3'
miRNA:   3'- -GGUUCa--UGC----GGAGACGGA---AAAGGCuG- -5'
27234 3' -51.1 NC_005832.1 + 48710 0.68 0.948208
Target:  5'- gCAAGUACGUCgaaGCC---UCCGACc -3'
miRNA:   3'- gGUUCAUGCGGagaCGGaaaAGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 58919 0.68 0.933481
Target:  5'- uCUcuGUugGCCUUUGCCUUUgCCa-- -3'
miRNA:   3'- -GGuuCAugCGGAGACGGAAAaGGcug -5'
27234 3' -51.1 NC_005832.1 + 54344 0.68 0.92804
Target:  5'- aCCAcGcgGCGCUggggGCCUUUUUCGGCg -3'
miRNA:   3'- -GGUuCa-UGCGGaga-CGGAAAAGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 33584 0.68 0.92804
Target:  5'- aCCugG-ACGCC-CUGCCUUUggCCGcCa -3'
miRNA:   3'- -GGuuCaUGCGGaGACGGAAAa-GGCuG- -5'
27234 3' -51.1 NC_005832.1 + 90283 0.69 0.922914
Target:  5'- gCCAAGcucuuggcauuuuuCGCCUCaGUCUUUcCCGACg -3'
miRNA:   3'- -GGUUCau------------GCGGAGaCGGAAAaGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 8437 0.7 0.889811
Target:  5'- uCCAcGUAC-UCUCUGCCguccgccUCCGGCa -3'
miRNA:   3'- -GGUuCAUGcGGAGACGGaaa----AGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 81007 0.7 0.875001
Target:  5'- uCCAagAGUACGCCUCgGCCUcc-CUGGu -3'
miRNA:   3'- -GGU--UCAUGCGGAGaCGGAaaaGGCUg -5'
27234 3' -51.1 NC_005832.1 + 13803 0.7 0.867228
Target:  5'- aCGAGUACGUCUCUGUg--UUCC-ACa -3'
miRNA:   3'- gGUUCAUGCGGAGACGgaaAAGGcUG- -5'
27234 3' -51.1 NC_005832.1 + 92629 0.72 0.784662
Target:  5'- -aAAGUAgaGCCUCUGCCUcUccucgucagaguagUCCGACu -3'
miRNA:   3'- ggUUCAUg-CGGAGACGGAaA--------------AGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 89740 0.74 0.673928
Target:  5'- uCCAAGUACGUCUUU-CCgg--CCGACa -3'
miRNA:   3'- -GGUUCAUGCGGAGAcGGaaaaGGCUG- -5'
27234 3' -51.1 NC_005832.1 + 97678 1.14 0.002926
Target:  5'- aCCAAGUACGCCUCUGCCUUUUCCGACg -3'
miRNA:   3'- -GGUUCAUGCGGAGACGGAAAAGGCUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.