Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27239 | 5' | -55.1 | NC_005832.1 | + | 56966 | 0.7 | 0.626958 |
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Target: 5'- -aGCGAGucucauGAAGGCCGUCGCcacUCAGAg- -3' miRNA: 3'- ugCGUUU------CUUCCGGCGGCG---AGUCUaa -5' |
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| 27239 | 5' | -55.1 | NC_005832.1 | + | 98307 | 0.73 | 0.451063 |
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Target: 5'- uGCGCAAGGGAGGCCGaaaggGcCUCGGGUc -3' miRNA: 3'- -UGCGUUUCUUCCGGCgg---C-GAGUCUAa -5' |
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| 27239 | 5' | -55.1 | NC_005832.1 | + | 38699 | 0.73 | 0.441491 |
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Target: 5'- gACGCAGAGAGGGuuGCggaGCUCgAGGa- -3' miRNA: 3'- -UGCGUUUCUUCCggCGg--CGAG-UCUaa -5' |
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| 27239 | 5' | -55.1 | NC_005832.1 | + | 42519 | 0.74 | 0.404396 |
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Target: 5'- gACGCGugcugcuccaugAGGAGGGCCGCCauGCUCuGGUg -3' miRNA: 3'- -UGCGU------------UUCUUCCGGCGG--CGAGuCUAa -5' |
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| 27239 | 5' | -55.1 | NC_005832.1 | + | 27856 | 0.74 | 0.404396 |
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Target: 5'- -gGCAAAGGAGGCgGCCGggCAGGc- -3' miRNA: 3'- ugCGUUUCUUCCGgCGGCgaGUCUaa -5' |
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| 27239 | 5' | -55.1 | NC_005832.1 | + | 41568 | 0.76 | 0.29113 |
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Target: 5'- cCGaGGAGGAGGCCGCCGCcgUCAGGg- -3' miRNA: 3'- uGCgUUUCUUCCGGCGGCG--AGUCUaa -5' |
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| 27239 | 5' | -55.1 | NC_005832.1 | + | 102308 | 1.05 | 0.003098 |
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Target: 5'- aACGCAAAGAAGGCCGCCGCUCAGAUUa -3' miRNA: 3'- -UGCGUUUCUUCCGGCGGCGAGUCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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