Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2724 | 3' | -56.7 | NC_001491.2 | + | 140164 | 0.66 | 0.881011 |
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Target: 5'- cCGCGCUccGugGGGGacuuugccgcggUCGUGcgCGCc -3' miRNA: 3'- aGCGCGG--UugCCCCa-----------AGCACuaGCGc -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 138780 | 0.67 | 0.860767 |
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Target: 5'- -gGUGUCuuCGGGGUUuucugcagacaCGUGcgCGCGg -3' miRNA: 3'- agCGCGGuuGCCCCAA-----------GCACuaGCGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 142453 | 0.67 | 0.860767 |
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Target: 5'- gCGCGCUcccgaGACGGGGg--GUGGgggCGUGg -3' miRNA: 3'- aGCGCGG-----UUGCCCCaagCACUa--GCGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 9451 | 0.68 | 0.829164 |
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Target: 5'- gCGCGCUcgaGGGGUUC---AUCGCGg -3' miRNA: 3'- aGCGCGGuugCCCCAAGcacUAGCGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 149180 | 0.68 | 0.803617 |
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Target: 5'- uUCGCGUCAgcagagggcgggGCGGGGUUCagcGUCaGCGg -3' miRNA: 3'- -AGCGCGGU------------UGCCCCAAGcacUAG-CGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 145298 | 0.71 | 0.670232 |
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Target: 5'- -gGCgGCCGACGGGGUUU----UCGCGg -3' miRNA: 3'- agCG-CGGUUGCCCCAAGcacuAGCGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 139979 | 0.72 | 0.609872 |
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Target: 5'- cCGCGCCGugGGGcGguaccUCGUGGaucucggCGCGg -3' miRNA: 3'- aGCGCGGUugCCC-Ca----AGCACUa------GCGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 6427 | 0.74 | 0.464315 |
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Target: 5'- cUCGCcUCGugGGGGUUUGUGGUgGCu -3' miRNA: 3'- -AGCGcGGUugCCCCAAGCACUAgCGc -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 38682 | 0.75 | 0.419772 |
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Target: 5'- cUCGUGCCAAUGaGGcGUUUGUGcgcGUCGCGc -3' miRNA: 3'- -AGCGCGGUUGC-CC-CAAGCAC---UAGCGC- -5' |
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| 2724 | 3' | -56.7 | NC_001491.2 | + | 143581 | 1.08 | 0.003158 |
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Target: 5'- gUCGCGCCAACGGGGUUCGUGAUCGCGc -3' miRNA: 3'- -AGCGCGGUUGCCCCAAGCACUAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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