Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27240 | 3' | -56.3 | NC_005832.1 | + | 67211 | 0.66 | 0.809399 |
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Target: 5'- cGGAGUGGcCUCCAgggccucggcgUCCAGG--UCAGg -3' miRNA: 3'- uUCUCACCaGAGGU-----------GGGUCCcaAGUC- -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 49474 | 0.66 | 0.790879 |
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Target: 5'- gAAGAGauguacggGGUCUCC-CUCAGGGcUCGu -3' miRNA: 3'- -UUCUCa-------CCAGAGGuGGGUCCCaAGUc -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 43112 | 0.66 | 0.781384 |
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Target: 5'- aGGGGGUGGgguUCUCCcuggagacACCCAGGGa---- -3' miRNA: 3'- -UUCUCACC---AGAGG--------UGGGUCCCaaguc -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 52305 | 0.67 | 0.742074 |
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Target: 5'- gGGGuAGUGGUCUacaaaCCACCCucccuugcuaGGGGcgCAGa -3' miRNA: 3'- -UUC-UCACCAGA-----GGUGGG----------UCCCaaGUC- -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 77436 | 0.67 | 0.731963 |
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Target: 5'- aGGGAG-GGUCUCC-CUCAGGGa---- -3' miRNA: 3'- -UUCUCaCCAGAGGuGGGUCCCaaguc -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 29650 | 0.67 | 0.731963 |
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Target: 5'- --uGGUGGcCUCCGCUCAGGGa---- -3' miRNA: 3'- uucUCACCaGAGGUGGGUCCCaaguc -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 69305 | 0.7 | 0.58519 |
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Target: 5'- cAGAGUGG-CUUCACCCAGGaGgUCu- -3' miRNA: 3'- uUCUCACCaGAGGUGGGUCC-CaAGuc -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 60283 | 0.71 | 0.53326 |
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Target: 5'- uGAGGGaagcgcuggGGUCUCUGCCCgAGGGUUCc- -3' miRNA: 3'- -UUCUCa--------CCAGAGGUGGG-UCCCAAGuc -5' |
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| 27240 | 3' | -56.3 | NC_005832.1 | + | 104839 | 1.07 | 0.002083 |
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Target: 5'- aAAGAGUGGUCUCCACCCAGGGUUCAGg -3' miRNA: 3'- -UUCUCACCAGAGGUGGGUCCCAAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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