miRNA: 3'- aAUCGAGGuaAUCcUUCUUGGAGUUc -5'
miRNA: 3'- aAUCGAGGua-AUCCU----------UCUUGGAGUUc -5'
miRNA: 3'- -AAUCGAGGUAAUCCUUCUUGGAGUUC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27247 | 3' | -47.4 | NC_005833.1 | + | 13977 | 0.69 | 0.89085 |
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Target: 5'- gUGGUUCCggUAGuAAGAGCCUUAAc -3' miRNA: 3'- aAUCGAGGuaAUCcUUCUUGGAGUUc -5' |
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| 27247 | 3' | -47.4 | NC_005833.1 | + | 25974 | 0.7 | 0.82844 |
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Target: 5'- aUGGUUCCugaUGGGAagggcgggacGGAACCUCGAu -3' miRNA: 3'- aAUCGAGGua-AUCCU----------UCUUGGAGUUc -5' |
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| 27247 | 3' | -47.4 | NC_005833.1 | + | 33619 | 1.06 | 0.006639 |
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Target: 5'- gUUAGCUCCAUUAGGAAGAACCUCAAGc -3' miRNA: 3'- -AAUCGAGGUAAUCCUUCUUGGAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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