Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27249 | 3' | -47.4 | NC_005839.2 | + | 102035 | 0.66 | 0.999316 |
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Target: 5'- aUGAUgcggGCCGAgCGGGUGAaaagagucgauCCAAAAAu- -3' miRNA: 3'- aACUA----CGGCU-GCCCAUU-----------GGUUUUUua -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 130968 | 0.66 | 0.999269 |
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Target: 5'- cUGAUGCCGAauuuaucaacgaacCGGGUGugUAu----- -3' miRNA: 3'- aACUACGGCU--------------GCCCAUugGUuuuuua -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 60792 | 0.66 | 0.999149 |
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Target: 5'- aUGAUGCCaa-GuGGUAGCUAAAAAu- -3' miRNA: 3'- aACUACGGcugC-CCAUUGGUUUUUua -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 97986 | 0.67 | 0.998084 |
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Target: 5'- cUGGUGgUGACGGGauuaaAAUCAAGAGAa -3' miRNA: 3'- aACUACgGCUGCCCa----UUGGUUUUUUa -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 31793 | 0.67 | 0.997688 |
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Target: 5'- aUUGGUGgCGuaaACGaGGUGACCGAAAu-- -3' miRNA: 3'- -AACUACgGC---UGC-CCAUUGGUUUUuua -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 116395 | 0.67 | 0.996069 |
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Target: 5'- aUGAUGCuaaCGGCGGGUc-UCAGAAAAg -3' miRNA: 3'- aACUACG---GCUGCCCAuuGGUUUUUUa -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 123604 | 0.73 | 0.918454 |
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Target: 5'- aUGAUGCCGAgGGG-GACguGAAAAa -3' miRNA: 3'- aACUACGGCUgCCCaUUGguUUUUUa -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 107868 | 0.78 | 0.689472 |
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Target: 5'- aUGAUGCCGACGGu--GCCAAAAu-- -3' miRNA: 3'- aACUACGGCUGCCcauUGGUUUUuua -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 89068 | 0.83 | 0.430235 |
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Target: 5'- gUGAUGCCGACuGGUGACuCAAAAGAa -3' miRNA: 3'- aACUACGGCUGcCCAUUG-GUUUUUUa -5' |
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| 27249 | 3' | -47.4 | NC_005839.2 | + | 123797 | 1.03 | 0.030569 |
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Target: 5'- gUUGAUGCCGACGGGUAACCAAAAAAUc -3' miRNA: 3'- -AACUACGGCUGCCCAUUGGUUUUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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