Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27253 | 5' | -50.9 | NC_005841.1 | + | 29774 | 0.66 | 0.868096 |
Target: 5'- -cGCAAACUGGcuaacaacGUugagcugaaagUCGCAaacauGGCCGCc -3' miRNA: 3'- gaUGUUUGACC--------UA-----------AGCGUgu---CCGGCG- -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 3346 | 0.66 | 0.859523 |
Target: 5'- -gGCAAGCUGGuua-GUuCAGGCUGg -3' miRNA: 3'- gaUGUUUGACCuaagCGuGUCCGGCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 9876 | 0.67 | 0.792677 |
Target: 5'- -cACAggUcGGcaUCGCGCAGGUCGg -3' miRNA: 3'- gaUGUuuGaCCuaAGCGUGUCCGGCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 10085 | 0.67 | 0.792677 |
Target: 5'- -cACAggUcGGcaUCGCGCAGGUCGg -3' miRNA: 3'- gaUGUuuGaCCuaAGCGUGUCCGGCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 9906 | 0.67 | 0.782284 |
Target: 5'- -cGCAggUUGGcacCGUACAGGUCGg -3' miRNA: 3'- gaUGUuuGACCuaaGCGUGUCCGGCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 10115 | 0.67 | 0.782284 |
Target: 5'- -cGCAggUUGGcacCGUACAGGUCGg -3' miRNA: 3'- gaUGUuuGACCuaaGCGUGUCCGGCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 29812 | 0.67 | 0.782284 |
Target: 5'- gCUGCGGACUGGAUUCGgugACGauacGCUGUc -3' miRNA: 3'- -GAUGUUUGACCUAAGCg--UGUc---CGGCG- -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 9846 | 0.68 | 0.750119 |
Target: 5'- -gGCAggUcGGcaUCGCGCAGGUCGg -3' miRNA: 3'- gaUGUuuGaCCuaAGCGUGUCCGGCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 34773 | 0.68 | 0.750119 |
Target: 5'- gCUGCAAGCUGGuuacuggUCGCuguagaaccaAGGCCa- -3' miRNA: 3'- -GAUGUUUGACCua-----AGCGug--------UCCGGcg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 9935 | 0.69 | 0.705425 |
Target: 5'- gUACAggUUGGcacCGCGCAGGuuGg -3' miRNA: 3'- gAUGUuuGACCuaaGCGUGUCCggCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 10039 | 0.69 | 0.705425 |
Target: 5'- gUACAggUUGGcacCGCGCAGGuuGg -3' miRNA: 3'- gAUGUuuGACCuaaGCGUGUCCggCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 9994 | 0.7 | 0.659451 |
Target: 5'- gUACAggUUGGcacCGCACAGGuuGg -3' miRNA: 3'- gAUGUuuGACCuaaGCGUGUCCggCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 7044 | 0.71 | 0.601429 |
Target: 5'- aCUugGAGCUaccGGAgcagUUGCGCGGGucauCCGCa -3' miRNA: 3'- -GAugUUUGA---CCUa---AGCGUGUCC----GGCG- -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 36502 | 0.72 | 0.510574 |
Target: 5'- aUGCAGGCUGuGAUUCcuGUACAGGuuGa -3' miRNA: 3'- gAUGUUUGAC-CUAAG--CGUGUCCggCg -5' |
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27253 | 5' | -50.9 | NC_005841.1 | + | 4466 | 1.07 | 0.002841 |
Target: 5'- -cACAAACUGGAUUCGCACAGGCCGCc -3' miRNA: 3'- gaUGUUUGACCUAAGCGUGUCCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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