Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27254 | 3' | -43.8 | NC_005841.1 | + | 26122 | 0.66 | 0.99861 |
Target: 5'- uGCUUGAAGGAAGAuuCAaaGCAuucaauACAuGCg -3' miRNA: 3'- -CGGACUUUCUUCUuuGU--UGU------UGU-CGg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 25739 | 0.66 | 0.998277 |
Target: 5'- uCCaGAuaccGGAAGAGACGAUuaagugcCAGCCg -3' miRNA: 3'- cGGaCUu---UCUUCUUUGUUGuu-----GUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 14076 | 0.67 | 0.996848 |
Target: 5'- cGCCUGAcgcuuuagcGAGAGcaccuuGAGACA-UAcCAGCCa -3' miRNA: 3'- -CGGACU---------UUCUU------CUUUGUuGUuGUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 33714 | 0.67 | 0.996848 |
Target: 5'- uGCUcGAcAGAccGGAugGACAGCAGCa -3' miRNA: 3'- -CGGaCUuUCUu-CUUugUUGUUGUCGg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 18146 | 0.68 | 0.993543 |
Target: 5'- uGCCaGAGAcGAAGGggGACGuauggcACGACAGCg -3' miRNA: 3'- -CGGaCUUU-CUUCU--UUGU------UGUUGUCGg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 18998 | 0.68 | 0.993543 |
Target: 5'- aGCagcAGAGAAGAAACGACGACGa-- -3' miRNA: 3'- -CGgacUUUCUUCUUUGUUGUUGUcgg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 35511 | 0.68 | 0.993543 |
Target: 5'- cCCUGAGA-AAG-AGCGACGuUAGCCu -3' miRNA: 3'- cGGACUUUcUUCuUUGUUGUuGUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 7635 | 0.68 | 0.992385 |
Target: 5'- uCCUGAAAGAcuucagcGAuuauGCAGCAACAGa- -3' miRNA: 3'- cGGACUUUCUu------CUu---UGUUGUUGUCgg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 36710 | 0.68 | 0.992385 |
Target: 5'- aGCCguUGGAGGuGGAuaAGCAGCAuagaaAGCCu -3' miRNA: 3'- -CGG--ACUUUCuUCU--UUGUUGUug---UCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 24798 | 0.68 | 0.991069 |
Target: 5'- aCCUGAuaAGGGAGAucGCGAUAAgcGCCg -3' miRNA: 3'- cGGACU--UUCUUCUu-UGUUGUUguCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 4900 | 0.69 | 0.98393 |
Target: 5'- cGCCUGAuuaucacaaaGGGAuauccGAAgcGCGACGAuucCAGCCg -3' miRNA: 3'- -CGGACU----------UUCUu----CUU--UGUUGUU---GUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 28859 | 0.7 | 0.972799 |
Target: 5'- aUCUGucaGAAGAAGGccuggccaaauauGACuauGACAACAGCCg -3' miRNA: 3'- cGGAC---UUUCUUCU-------------UUG---UUGUUGUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 4185 | 0.7 | 0.96975 |
Target: 5'- -aCUGAAGGcAGAAGCAugGAgGGUa -3' miRNA: 3'- cgGACUUUCuUCUUUGUugUUgUCGg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 36584 | 0.71 | 0.967587 |
Target: 5'- uGCCUGGuacuuuuGAGAGGCAAUAACgucuuuccaugccuuAGCCa -3' miRNA: 3'- -CGGACUuuc----UUCUUUGUUGUUG---------------UCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 26803 | 0.71 | 0.964139 |
Target: 5'- uGCCUGcucccauguuaucuGGGACAGCAACAGCa -3' miRNA: 3'- -CGGACuuucuu--------CUUUGUUGUUGUCGg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 28065 | 0.71 | 0.957838 |
Target: 5'- aGUgUGAAGuacuGAAGCAACAGCAGUUg -3' miRNA: 3'- -CGgACUUUcuu-CUUUGUUGUUGUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 19432 | 0.72 | 0.943059 |
Target: 5'- uGCgUGAcagcAGAAGAGagGCAGCAugAGCa -3' miRNA: 3'- -CGgACUu---UCUUCUU--UGUUGUugUCGg -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 8275 | 0.72 | 0.936888 |
Target: 5'- uCCUGAAauugaGGAAGAAguggaGCAGCAAauuaacuUAGCCg -3' miRNA: 3'- cGGACUU-----UCUUCUU-----UGUUGUU-------GUCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 32463 | 0.73 | 0.911691 |
Target: 5'- cGCCUGucAGGGAGAAAUAcucuaucguCGACgAGCCa -3' miRNA: 3'- -CGGACu-UUCUUCUUUGUu--------GUUG-UCGG- -5' |
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27254 | 3' | -43.8 | NC_005841.1 | + | 14794 | 0.73 | 0.888817 |
Target: 5'- aCCUGAGAGAAGAAACcguucuucCAuCGGCUu -3' miRNA: 3'- cGGACUUUCUUCUUUGuu------GUuGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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