miRNA: 3'- uAGACUaCGau--GUg---GUUCGAAUGCGU- -5'
miRNA: 3'- uaGACUACGAUGUGGUUCGaaugCGU- -5'
miRNA: 3'- -UAGACUACGAUGugG--UUCGAau---GCGU- -5'
miRNA: 3'- -UAGACU----------ACGAUGUGGUuCGAaUGCGU- -5'
miRNA: 3'- -UAGACUACGAUGUGGUUCGaAUGCGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27260 | 5' | -49.9 | NC_005841.1 | + | 26611 | 0.66 | 0.867588 |
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Target: 5'- uUCUGAcGCaucuCAgaaaCGAGCUUGCGCAc -3' miRNA: 3'- uAGACUaCGau--GUg---GUUCGAAUGCGU- -5' |
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| 27260 | 5' | -49.9 | NC_005841.1 | + | 37153 | 0.66 | 0.84022 |
|
Target: 5'- -aCUGGUGUUGCAUUAGGCggg-GCAa -3' miRNA: 3'- uaGACUACGAUGUGGUUCGaaugCGU- -5' |
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| 27260 | 5' | -49.9 | NC_005841.1 | + | 34945 | 0.69 | 0.711061 |
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Target: 5'- cGUCUGGUGCUAUugCugGGGCUggacuUGCGa -3' miRNA: 3'- -UAGACUACGAUGugG--UUCGAau---GCGU- -5' |
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| 27260 | 5' | -49.9 | NC_005841.1 | + | 22157 | 0.71 | 0.580321 |
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Target: 5'- uAUCUGGcgucaaguucUGCUACAUCAcGCUgGCGCGu -3' miRNA: 3'- -UAGACU----------ACGAUGUGGUuCGAaUGCGU- -5' |
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| 27260 | 5' | -49.9 | NC_005841.1 | + | 31431 | 0.98 | 0.010721 |
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Target: 5'- cAUCUGAUGCUACACCAAGC-UACGCAg -3' miRNA: 3'- -UAGACUACGAUGUGGUUCGaAUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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