Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27262 | 5' | -46.2 | NC_005841.1 | + | 8959 | 0.66 | 0.982747 |
Target: 5'- aGGAac-UUGCgGGAU--AUGCCAGaCCg -3' miRNA: 3'- -CCUauaAACGgUCUAauUGCGGUC-GG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 22111 | 0.67 | 0.980347 |
Target: 5'- cGGUAaUUGUCGcGUUcgccAGCGCCAGCUu -3' miRNA: 3'- cCUAUaAACGGUcUAA----UUGCGGUCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 2041 | 0.67 | 0.976581 |
Target: 5'- -------aGCCAGcaccauauccGCGCCAGCCu -3' miRNA: 3'- ccuauaaaCGGUCuaau------UGCGGUCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 5460 | 0.67 | 0.974814 |
Target: 5'- cGGAg--UUGCCGGAU-AGCuGCCcgauAGCUc -3' miRNA: 3'- -CCUauaAACGGUCUAaUUG-CGG----UCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 27154 | 0.67 | 0.971656 |
Target: 5'- -------cGCCAGAUUAAgCGUC-GCCg -3' miRNA: 3'- ccuauaaaCGGUCUAAUU-GCGGuCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 37720 | 0.67 | 0.968223 |
Target: 5'- aGGAUGgaUGUCAGAggagaaagGAcacaaaugccacCGCCAGCUg -3' miRNA: 3'- -CCUAUaaACGGUCUaa------UU------------GCGGUCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 2098 | 0.68 | 0.964505 |
Target: 5'- cGGAUAUggUGCUGGcuaAGCGCuCAGaCCg -3' miRNA: 3'- -CCUAUAa-ACGGUCuaaUUGCG-GUC-GG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 33725 | 0.7 | 0.917032 |
Target: 5'- aGGAcg-UUGUCAcguuGAgaggUGACGCCAGCg -3' miRNA: 3'- -CCUauaAACGGU----CUa---AUUGCGGUCGg -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 34202 | 0.7 | 0.917032 |
Target: 5'- -------cGCCAGA---ACGUCAGCCc -3' miRNA: 3'- ccuauaaaCGGUCUaauUGCGGUCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 28844 | 0.72 | 0.834142 |
Target: 5'- cGGAUAUUaaugccuaucUGUCAGAagAAgGCCuGGCCa -3' miRNA: 3'- -CCUAUAA----------ACGGUCUaaUUgCGG-UCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 6556 | 0.72 | 0.834142 |
Target: 5'- cGGAg----GCCaAGAUUuccGCGCUGGCCg -3' miRNA: 3'- -CCUauaaaCGG-UCUAAu--UGCGGUCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 36183 | 0.72 | 0.834142 |
Target: 5'- -----aUUGCCGGAUUuccugcucCGCCAGUCa -3' miRNA: 3'- ccuauaAACGGUCUAAuu------GCGGUCGG- -5' |
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27262 | 5' | -46.2 | NC_005841.1 | + | 36844 | 1.16 | 0.002171 |
Target: 5'- gGGAUAUUUGCCAGAUUAACGCCAGCCu -3' miRNA: 3'- -CCUAUAAACGGUCUAAUUGCGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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