Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27264 | 3' | -59.3 | NC_005841.1 | + | 10136 | 0.74 | 0.129954 |
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Target: 5'- uCGGCACCGUaCAGGUUGGCucuagauCCGc-- -3' miRNA: 3'- -GCCGUGGCGcGUCCAACCGu------GGCaug -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 10076 | 0.8 | 0.040126 |
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Target: 5'- uCGGCACCGCaCAGGUcGGCAUCGcGCa -3' miRNA: 3'- -GCCGUGGCGcGUCCAaCCGUGGCaUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 9852 | 0.9 | 0.007016 |
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Target: 5'- uCGGCAUCGCGCAGGUcGGCACCGcACa -3' miRNA: 3'- -GCCGUGGCGCGUCCAaCCGUGGCaUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 9973 | 0.97 | 0.002008 |
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Target: 5'- uGGCACCG-GCAGGUUGGCACCGUACa -3' miRNA: 3'- gCCGUGGCgCGUCCAACCGUGGCAUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 10002 | 0.98 | 0.001949 |
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Target: 5'- uGGCACCGCaCAGGUUGGCACCGUACa -3' miRNA: 3'- gCCGUGGCGcGUCCAACCGUGGCAUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 9943 | 1.08 | 0.000307 |
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Target: 5'- uGGCACCGCGCAGGUUGGCACCGUACa -3' miRNA: 3'- gCCGUGGCGCGUCCAACCGUGGCAUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 10047 | 1.08 | 0.000307 |
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Target: 5'- uGGCACCGCGCAGGUUGGCACCGUACa -3' miRNA: 3'- gCCGUGGCGCGUCCAACCGUGGCAUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 9897 | 1.09 | 0.000242 |
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Target: 5'- uCGGCACCGCGCAGGUUGGCACCGUACa -3' miRNA: 3'- -GCCGUGGCGCGUCCAACCGUGGCAUG- -5' |
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| 27264 | 3' | -59.3 | NC_005841.1 | + | 10106 | 1.09 | 0.000242 |
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Target: 5'- uCGGCACCGCGCAGGUUGGCACCGUACa -3' miRNA: 3'- -GCCGUGGCGCGUCCAACCGUGGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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