Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27264 | 5' | -57 | NC_005841.1 | + | 9846 | 0.8 | 0.06799 |
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Target: 5'- ---gGCAGGUcGGCAUCGCGCAGGUc -3' miRNA: 3'- ggcaUGUCCAaCCGUGGCGCGUCCAa -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 10126 | 0.87 | 0.018895 |
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Target: 5'- aCCGUACAGGUcGGCACCGUaCAGGUUg -3' miRNA: 3'- -GGCAUGUCCAaCCGUGGCGcGUCCAA- -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 10066 | 0.88 | 0.016752 |
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Target: 5'- aCCGUACAGGUcGGCACCGCaCAGGUc -3' miRNA: 3'- -GGCAUGUCCAaCCGUGGCGcGUCCAa -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 9887 | 0.9 | 0.012023 |
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Target: 5'- aUCGcGCAGGUcGGCACCGCGCAGGUUg -3' miRNA: 3'- -GGCaUGUCCAaCCGUGGCGCGUCCAA- -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 10096 | 0.9 | 0.012023 |
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Target: 5'- aUCGcGCAGGUcGGCACCGCGCAGGUUg -3' miRNA: 3'- -GGCaUGUCCAaCCGUGGCGCGUCCAA- -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 9962 | 0.97 | 0.003476 |
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Target: 5'- aCCGUACAGGUUGGCACCG-GCAGGUUg -3' miRNA: 3'- -GGCAUGUCCAACCGUGGCgCGUCCAA- -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 9991 | 0.97 | 0.003372 |
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Target: 5'- aCCGUACAGGUUGGCACCGCaCAGGUUg -3' miRNA: 3'- -GGCAUGUCCAACCGUGGCGcGUCCAA- -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 9932 | 1.08 | 0.000514 |
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Target: 5'- aCCGUACAGGUUGGCACCGCGCAGGUUg -3' miRNA: 3'- -GGCAUGUCCAACCGUGGCGCGUCCAA- -5' |
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| 27264 | 5' | -57 | NC_005841.1 | + | 10036 | 1.08 | 0.000514 |
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Target: 5'- aCCGUACAGGUUGGCACCGCGCAGGUUg -3' miRNA: 3'- -GGCAUGUCCAACCGUGGCGCGUCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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