Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27265 | 3' | -57.5 | NC_005841.1 | + | 10092 | 0.9 | 0.010478 |
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Target: 5'- cGGCAUCGcGCAGGUcGGCACCGCGCa -3' miRNA: 3'- aCCGUGGCaUGUCCAaCCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 9853 | 0.9 | 0.009283 |
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Target: 5'- cGGCAUCGcGCAGGUcGGCACCGCACa -3' miRNA: 3'- aCCGUGGCaUGUCCAaCCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 9957 | 0.98 | 0.0023 |
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Target: 5'- uUGGCACCGUACAGGUUGGCACCgGCAg -3' miRNA: 3'- -ACCGUGGCAUGUCCAACCGUGG-CGUg -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 9898 | 0.98 | 0.002231 |
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Target: 5'- cGGCACCGcGCAGGUUGGCACCGUACa -3' miRNA: 3'- aCCGUGGCaUGUCCAACCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 10121 | 0.99 | 0.001916 |
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Target: 5'- uUGGCACCGUACAGGUcGGCACCGUACa -3' miRNA: 3'- -ACCGUGGCAUGUCCAaCCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 10061 | 1.03 | 0.001043 |
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Target: 5'- uUGGCACCGUACAGGUcGGCACCGCACa -3' miRNA: 3'- -ACCGUGGCAUGUCCAaCCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 9928 | 1.09 | 0.000371 |
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Target: 5'- cGGCACCGUACAGGUUGGCACCGCGCa -3' miRNA: 3'- aCCGUGGCAUGUCCAACCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 10031 | 1.09 | 0.000339 |
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Target: 5'- uUGGCACCGUACAGGUUGGCACCGCGCa -3' miRNA: 3'- -ACCGUGGCAUGUCCAACCGUGGCGUG- -5' |
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| 27265 | 3' | -57.5 | NC_005841.1 | + | 9986 | 1.1 | 0.0003 |
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Target: 5'- uUGGCACCGUACAGGUUGGCACCGCACa -3' miRNA: 3'- -ACCGUGGCAUGUCCAACCGUGGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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