Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27265 | 5' | -53.5 | NC_005841.1 | + | 31775 | 0.66 | 0.718518 |
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Target: 5'- aCCGcUGCcuGUUGGCAUgCGUgACGGGUa -3' miRNA: 3'- -GGC-AUGucCAACCGUG-GCA-UGUCCAa -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 9857 | 0.78 | 0.155436 |
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Target: 5'- aUCGcGCAGGUcGGCACCGcACAGGUc -3' miRNA: 3'- -GGCaUGUCCAaCCGUGGCaUGUCCAa -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 9887 | 0.81 | 0.100668 |
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Target: 5'- aUCGcGCAGGUcGGCACCGcGCAGGUUg -3' miRNA: 3'- -GGCaUGUCCAaCCGUGGCaUGUCCAA- -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 10096 | 0.81 | 0.100668 |
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Target: 5'- aUCGcGCAGGUcGGCACCGcGCAGGUUg -3' miRNA: 3'- -GGCaUGUCCAaCCGUGGCaUGUCCAA- -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 10051 | 0.98 | 0.006103 |
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Target: 5'- aCCGcGCAGGUUGGCACCGUACAGGUc -3' miRNA: 3'- -GGCaUGUCCAACCGUGGCAUGUCCAa -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 9991 | 1 | 0.00462 |
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Target: 5'- aCCGUACAGGUUGGCACCGcACAGGUUg -3' miRNA: 3'- -GGCAUGUCCAACCGUGGCaUGUCCAA- -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 9947 | 1 | 0.004211 |
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Target: 5'- aCCGcGCAGGUUGGCACCGUACAGGUUg -3' miRNA: 3'- -GGCaUGUCCAACCGUGGCAUGUCCAA- -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 9917 | 1.01 | 0.003958 |
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Target: 5'- aCCGUACAGGUcGGCACCGUACAGGUUg -3' miRNA: 3'- -GGCAUGUCCAaCCGUGGCAUGUCCAA- -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 10126 | 1.01 | 0.003958 |
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Target: 5'- aCCGUACAGGUcGGCACCGUACAGGUUg -3' miRNA: 3'- -GGCAUGUCCAaCCGUGGCAUGUCCAA- -5' |
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| 27265 | 5' | -53.5 | NC_005841.1 | + | 10021 | 1.08 | 0.001112 |
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Target: 5'- aCCGUACAGGUUGGCACCGUACAGGUUg -3' miRNA: 3'- -GGCAUGUCCAACCGUGGCAUGUCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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