Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27266 | 3' | -48.5 | NC_005841.1 | + | 27315 | 0.66 | 0.923911 |
Target: 5'- aGCCCcacUCuCCGAaUACCGGAACAa- -3' miRNA: 3'- -CGGGau-AGuGGUUaGUGGUCUUGUau -5' |
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27266 | 3' | -48.5 | NC_005841.1 | + | 21653 | 0.68 | 0.862911 |
Target: 5'- aCCUUAUCACU-GUCGCCAG-ACAa- -3' miRNA: 3'- cGGGAUAGUGGuUAGUGGUCuUGUau -5' |
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27266 | 3' | -48.5 | NC_005841.1 | + | 36820 | 0.7 | 0.773278 |
Target: 5'- uGCa--GUCGCCAG-CGCCGGGGCAUGg -3' miRNA: 3'- -CGggaUAGUGGUUaGUGGUCUUGUAU- -5' |
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27266 | 3' | -48.5 | NC_005841.1 | + | 21073 | 0.72 | 0.622222 |
Target: 5'- cGCCCUA-C-CCAGUCGCCAcgaauGAGCAa- -3' miRNA: 3'- -CGGGAUaGuGGUUAGUGGU-----CUUGUau -5' |
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27266 | 3' | -48.5 | NC_005841.1 | + | 20966 | 1.1 | 0.002569 |
Target: 5'- cGCCCUAUCACCAAUCACCAGAACAUAu -3' miRNA: 3'- -CGGGAUAGUGGUUAGUGGUCUUGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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