Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27267 | 5' | -48.8 | NC_005841.1 | + | 3635 | 0.66 | 0.937025 |
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Target: 5'- uGGCAGCAgauagacauGCAGCGAAaggUGGcaUGGAUAc -3' miRNA: 3'- cCUGUCGU---------UGUCGUUUg--ACU--ACCUGU- -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 35410 | 0.66 | 0.931251 |
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Target: 5'- ---gAGCAGCAGCGuAACgauAUGGGCGa -3' miRNA: 3'- ccugUCGUUGUCGU-UUGac-UACCUGU- -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 36232 | 0.66 | 0.91874 |
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Target: 5'- aGGGCguGGCAGCAGCAAGagauAUGGGu- -3' miRNA: 3'- -CCUG--UCGUUGUCGUUUgac-UACCUgu -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 7781 | 0.68 | 0.856213 |
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Target: 5'- aGGAUGGCAcAgGGCAAAaCUGAaGGAUg -3' miRNA: 3'- -CCUGUCGU-UgUCGUUU-GACUaCCUGu -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 23242 | 0.68 | 0.847088 |
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Target: 5'- aGAUucuuuGUggUGGCGGACUGAUGGAa- -3' miRNA: 3'- cCUGu----CGuuGUCGUUUGACUACCUgu -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 31066 | 0.69 | 0.818184 |
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Target: 5'- uGGAgcuuuCAGCAucaugACGGCGAGCaauggGAUGGAUg -3' miRNA: 3'- -CCU-----GUCGU-----UGUCGUUUGa----CUACCUGu -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 27612 | 0.7 | 0.765652 |
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Target: 5'- -uGCAGaGGCGGUAAAC-GGUGGACAg -3' miRNA: 3'- ccUGUCgUUGUCGUUUGaCUACCUGU- -5' |
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| 27267 | 5' | -48.8 | NC_005841.1 | + | 26823 | 1.12 | 0.001831 |
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Target: 5'- gGGACAGCAACAGCAAACUGAUGGACAa -3' miRNA: 3'- -CCUGUCGUUGUCGUUUGACUACCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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