Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27270 | 3' | -50.1 | NC_005841.1 | + | 8440 | 0.66 | 0.865066 |
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Target: 5'- aCAGGCGUAaugGUCauGGCGUGGUAcUCCu- -3' miRNA: 3'- -GUUCGCGU---UAG--UCGCGUCAUaAGGug -5' |
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| 27270 | 3' | -50.1 | NC_005841.1 | + | 32417 | 0.67 | 0.8377 |
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Target: 5'- aCAGGCGCAaccaccGUUGGCGCugcguuGUAUgUCGCg -3' miRNA: 3'- -GUUCGCGU------UAGUCGCGu-----CAUAaGGUG- -5' |
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| 27270 | 3' | -50.1 | NC_005841.1 | + | 32381 | 0.67 | 0.8377 |
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Target: 5'- uCAGGCuGCGAuUCuGCGCGaUAUUCUGCa -3' miRNA: 3'- -GUUCG-CGUU-AGuCGCGUcAUAAGGUG- -5' |
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| 27270 | 3' | -50.1 | NC_005841.1 | + | 29294 | 0.67 | 0.8377 |
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Target: 5'- uGAGUuCAAUCAGCGCGGacugcccaUCCAUc -3' miRNA: 3'- gUUCGcGUUAGUCGCGUCaua-----AGGUG- -5' |
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| 27270 | 3' | -50.1 | NC_005841.1 | + | 35004 | 0.68 | 0.754612 |
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Target: 5'- gGGGCuGCGAUCGGCGgCGGUcuuggUCUGCu -3' miRNA: 3'- gUUCG-CGUUAGUCGC-GUCAua---AGGUG- -5' |
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| 27270 | 3' | -50.1 | NC_005841.1 | + | 31553 | 1.11 | 0.001462 |
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Target: 5'- uCAAGCGCAAUCAGCGCAGUAUUCCACa -3' miRNA: 3'- -GUUCGCGUUAGUCGCGUCAUAAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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