Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27271 | 5' | -51.5 | NC_005841.1 | + | 22107 | 0.66 | 0.835233 |
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Target: 5'- aCGCCAGAuacaCAAAGGAGCUUgCUgaUGCg -3' miRNA: 3'- -GCGGUCUcg--GUUUUCUCGAG-GA--AUGg -5' |
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| 27271 | 5' | -51.5 | NC_005841.1 | + | 14675 | 0.66 | 0.832412 |
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Target: 5'- gGCCAGAagacauucgcuaaaGCUAugGGuGUUCCUgaaUACCa -3' miRNA: 3'- gCGGUCU--------------CGGUuuUCuCGAGGA---AUGG- -5' |
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| 27271 | 5' | -51.5 | NC_005841.1 | + | 27453 | 0.67 | 0.753699 |
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Target: 5'- uGCuCAG-GCCAGAAGAaagGCUUCUUcuuugggggcggGCCa -3' miRNA: 3'- gCG-GUCuCGGUUUUCU---CGAGGAA------------UGG- -5' |
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| 27271 | 5' | -51.5 | NC_005841.1 | + | 40736 | 0.67 | 0.749336 |
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Target: 5'- gCGCCAGAGCCAGcgcaaucuucuacgGuaauauuAGAcacauacauGC-CCUUACCa -3' miRNA: 3'- -GCGGUCUCGGUU--------------U-------UCU---------CGaGGAAUGG- -5' |
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| 27271 | 5' | -51.5 | NC_005841.1 | + | 6040 | 0.68 | 0.69785 |
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Target: 5'- cCGCCAGAGCCAuauucAGGGUgaucugCUUcGCUg -3' miRNA: 3'- -GCGGUCUCGGUuu---UCUCGa-----GGAaUGG- -5' |
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| 27271 | 5' | -51.5 | NC_005841.1 | + | 31683 | 1.12 | 0.001024 |
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Target: 5'- uCGCCAGAGCCAAAAGAGCUCCUUACCc -3' miRNA: 3'- -GCGGUCUCGGUUUUCUCGAGGAAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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