Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27285 | 3' | -50 | NC_005856.1 | + | 31106 | 0.66 | 0.967537 |
Target: 5'- uGCAGUgaCAGcUGGCGCggUGGCGUa-- -3' miRNA: 3'- cCGUUA--GUC-ACCGUGaaACCGUAcuu -5' |
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27285 | 3' | -50 | NC_005856.1 | + | 37013 | 0.67 | 0.953579 |
Target: 5'- uGGCGuuccuGUCAGacucgggcuuucuauUGGCGCUUUGGUAUc-- -3' miRNA: 3'- -CCGU-----UAGUC---------------ACCGUGAAACCGUAcuu -5' |
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27285 | 3' | -50 | NC_005856.1 | + | 17474 | 0.7 | 0.87759 |
Target: 5'- gGGCGAU-AGUGGCAUggcUGGCgAUGGg -3' miRNA: 3'- -CCGUUAgUCACCGUGaa-ACCG-UACUu -5' |
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27285 | 3' | -50 | NC_005856.1 | + | 84559 | 0.7 | 0.861329 |
Target: 5'- aGGUuuUCAGUGGUACgg-GGC-UGAu -3' miRNA: 3'- -CCGuuAGUCACCGUGaaaCCGuACUu -5' |
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27285 | 3' | -50 | NC_005856.1 | + | 29550 | 0.73 | 0.712996 |
Target: 5'- cGCGAUCGGcGGCGCUgaaaUUGGCcgGGc -3' miRNA: 3'- cCGUUAGUCaCCGUGA----AACCGuaCUu -5' |
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27285 | 3' | -50 | NC_005856.1 | + | 8358 | 1.1 | 0.004598 |
Target: 5'- aGGCAAUCAGUGGCACUUUGGCAUGAAg -3' miRNA: 3'- -CCGUUAGUCACCGUGAAACCGUACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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