Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27286 | 5' | -54 | NC_005856.1 | + | 85739 | 0.72 | 0.569055 |
Target: 5'- uAUGCCGCCAUCAagGcGGCugCUAa -3' miRNA: 3'- cUACGGUGGUAGUaaCuCCGugGGUc -5' |
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27286 | 5' | -54 | NC_005856.1 | + | 16968 | 0.67 | 0.809804 |
Target: 5'- aAUGCCGCCuUCAccGGuGUACCCAGc -3' miRNA: 3'- cUACGGUGGuAGUaaCUcCGUGGGUC- -5' |
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27286 | 5' | -54 | NC_005856.1 | + | 42190 | 0.66 | 0.885217 |
Target: 5'- --aGUUGCCAUUAUUGuAGGUAUCCGc -3' miRNA: 3'- cuaCGGUGGUAGUAAC-UCCGUGGGUc -5' |
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27286 | 5' | -54 | NC_005856.1 | + | 16384 | 0.73 | 0.495729 |
Target: 5'- --gGCCGCCGgu-UUGuucauGGCACCCAGg -3' miRNA: 3'- cuaCGGUGGUaguAACu----CCGUGGGUC- -5' |
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27286 | 5' | -54 | NC_005856.1 | + | 8439 | 1.08 | 0.002435 |
Target: 5'- cGAUGCCACCAUCAUUGAGGCACCCAGc -3' miRNA: 3'- -CUACGGUGGUAGUAACUCCGUGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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