miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27287 5' -45.1 NC_005856.1 + 49404 0.66 0.999807
Target:  5'- gGCAGCCAggcguaGAGC-UUcUGGUAAUGu -3'
miRNA:   3'- aUGUCGGUa-----CUCGcAAaACUAUUACc -5'
27287 5' -45.1 NC_005856.1 + 54496 0.66 0.999749
Target:  5'- -uCAGCCAggGAGCGgcaaUGAacAUGGc -3'
miRNA:   3'- auGUCGGUa-CUCGCaaa-ACUauUACC- -5'
27287 5' -45.1 NC_005856.1 + 62579 0.68 0.998712
Target:  5'- gUGCAGCC-UGuAGCGU--UGAUGcgcuUGGg -3'
miRNA:   3'- -AUGUCGGuAC-UCGCAaaACUAUu---ACC- -5'
27287 5' -45.1 NC_005856.1 + 23237 0.68 0.998418
Target:  5'- aUAUGGCCAUGAaugGCGUUg-GAUGccGGg -3'
miRNA:   3'- -AUGUCGGUACU---CGCAAaaCUAUuaCC- -5'
27287 5' -45.1 NC_005856.1 + 82562 0.68 0.998069
Target:  5'- cGCGGCCcgGA-UGUUcugGAUAAUGGc -3'
miRNA:   3'- aUGUCGGuaCUcGCAAaa-CUAUUACC- -5'
27287 5' -45.1 NC_005856.1 + 19054 0.71 0.986098
Target:  5'- uUGC-GCCAguuccugUGgcGGUGUUUUGAUGAUGGa -3'
miRNA:   3'- -AUGuCGGU-------AC--UCGCAAAACUAUUACC- -5'
27287 5' -45.1 NC_005856.1 + 10226 1.1 0.016499
Target:  5'- cUACAGCCAUGAGCGUUUUGAUAAUGGu -3'
miRNA:   3'- -AUGUCGGUACUCGCAAAACUAUUACC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.