Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27289 | 3' | -50.3 | NC_005856.1 | + | 64652 | 0.66 | 0.970577 |
Target: 5'- cACCGGCGGCGGCcaauuuuUUAuauGCGGauaacuGGUAGUc -3' miRNA: 3'- -UGGUCGCCGCUGu------AAU---UGCU------CUAUCA- -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 20804 | 0.67 | 0.967343 |
Target: 5'- cCCGGCGGgGGUAUggacUGACGGGAUcGGUa -3' miRNA: 3'- uGGUCGCCgCUGUA----AUUGCUCUA-UCA- -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 58515 | 0.68 | 0.932199 |
Target: 5'- uUgAGCGGUGGCAUUAucaGAGAUAc- -3' miRNA: 3'- uGgUCGCCGCUGUAAUug-CUCUAUca -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 2893 | 0.7 | 0.863752 |
Target: 5'- cACCAGCGGCGGC-UUAGCaauacaacuGaAGGUAGc -3' miRNA: 3'- -UGGUCGCCGCUGuAAUUG---------C-UCUAUCa -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 17080 | 0.73 | 0.751007 |
Target: 5'- cACCGGUgaaGGCGGCAUUGGCGGGuccGUc -3' miRNA: 3'- -UGGUCG---CCGCUGUAAUUGCUCuauCA- -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 65861 | 0.73 | 0.719335 |
Target: 5'- uGCCAGCGGCGGCAggca-GAGGa--- -3' miRNA: 3'- -UGGUCGCCGCUGUaauugCUCUauca -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 66576 | 0.73 | 0.708588 |
Target: 5'- gGCCAGCGGUGcGCAagAACGgcAGAUGGa -3' miRNA: 3'- -UGGUCGCCGC-UGUaaUUGC--UCUAUCa -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 55301 | 0.74 | 0.697765 |
Target: 5'- aACCGGCGGC-ACAgaUUAACGAGAa--- -3' miRNA: 3'- -UGGUCGCCGcUGU--AAUUGCUCUauca -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 38845 | 0.76 | 0.555108 |
Target: 5'- uACCGGCGGC-ACAUUcGGCGAGAUGu- -3' miRNA: 3'- -UGGUCGCCGcUGUAA-UUGCUCUAUca -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 71959 | 0.79 | 0.422024 |
Target: 5'- gGCgGGCGGCGACAaUGAUGAGAUcGGa -3' miRNA: 3'- -UGgUCGCCGCUGUaAUUGCUCUA-UCa -5' |
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27289 | 3' | -50.3 | NC_005856.1 | + | 15450 | 1.08 | 0.006427 |
Target: 5'- cACCAGCGGCGACAUUAACGAGAUAGUg -3' miRNA: 3'- -UGGUCGCCGCUGUAAUUGCUCUAUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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