Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27289 | 5' | -54.1 | NC_005856.1 | + | 69246 | 0.66 | 0.907781 |
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Target: 5'- uGGAAgcCCUCGCCggaaACGUaC-CCGGCa -3' miRNA: 3'- -CCUUaaGGAGCGG----UGCGaGuGGCUGc -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 75442 | 0.66 | 0.905877 |
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Target: 5'- cGGAGcUUCCUauCCugGCUuauaugcagcaaacCACCGGCa -3' miRNA: 3'- -CCUU-AAGGAgcGGugCGA--------------GUGGCUGc -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 90170 | 0.66 | 0.90069 |
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Target: 5'- uGAAUUCUUUcgccguaGCCGCGCUCcgcGCCGGu- -3' miRNA: 3'- cCUUAAGGAG-------CGGUGCGAG---UGGCUgc -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 27713 | 0.67 | 0.88774 |
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Target: 5'- ----cUCCUCGCCACuaccgUCGCCaGACGc -3' miRNA: 3'- ccuuaAGGAGCGGUGcg---AGUGG-CUGC- -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 69592 | 0.67 | 0.879846 |
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Target: 5'- aGGGAUUcgaacCCUCuGCCAaGCUCGgcgaucuCCGACGu -3' miRNA: 3'- -CCUUAA-----GGAG-CGGUgCGAGU-------GGCUGC- -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 24762 | 0.69 | 0.778184 |
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Target: 5'- --cAUUCaggCUUGCCACGCcCACCGAUa -3' miRNA: 3'- ccuUAAG---GAGCGGUGCGaGUGGCUGc -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 15473 | 0.69 | 0.768529 |
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Target: 5'- gGGAuuGUUCCguUCGcCCGCGC-CACCaGCGg -3' miRNA: 3'- -CCU--UAAGG--AGC-GGUGCGaGUGGcUGC- -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 15573 | 0.7 | 0.748841 |
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Target: 5'- -uGAUUUC-CGCCuuGCGUUUACCGGCGa -3' miRNA: 3'- ccUUAAGGaGCGG--UGCGAGUGGCUGC- -5' |
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| 27289 | 5' | -54.1 | NC_005856.1 | + | 15412 | 1.12 | 0.001823 |
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Target: 5'- aGGAAUUCCUCGCCACGCUCACCGACGg -3' miRNA: 3'- -CCUUAAGGAGCGGUGCGAGUGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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