Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27291 | 3' | -50.6 | NC_005856.1 | + | 10903 | 0.67 | 0.949903 |
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Target: 5'- cACAUGAUucuUGCgCAugcugagcaauUCCGGCGuaACCu -3' miRNA: 3'- -UGUACUAuuuACGgGU-----------AGGCCGC--UGG- -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 2725 | 0.68 | 0.9453 |
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Target: 5'- uGCAUGAUGuuUGCCaucUCauaGGCGucaGCCa -3' miRNA: 3'- -UGUACUAUuuACGGgu-AGg--CCGC---UGG- -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 84974 | 0.68 | 0.935285 |
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Target: 5'- uGCGUGA----UGCCCAUCgCGGCa--- -3' miRNA: 3'- -UGUACUauuuACGGGUAG-GCCGcugg -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 27850 | 0.7 | 0.876775 |
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Target: 5'- aACA-GAUGGGguacgGCgCGUCUGGCGACg -3' miRNA: 3'- -UGUaCUAUUUa----CGgGUAGGCCGCUGg -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 19175 | 0.7 | 0.876775 |
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Target: 5'- uACAUacUGuc-GCCCGuUCUGGCGACCu -3' miRNA: 3'- -UGUAcuAUuuaCGGGU-AGGCCGCUGG- -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 49582 | 0.74 | 0.684532 |
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Target: 5'- cCAUGA-GAAUGCCguUCUGGCGcaACCa -3' miRNA: 3'- uGUACUaUUUACGGguAGGCCGC--UGG- -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 28657 | 0.76 | 0.564499 |
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Target: 5'- uGCAUaucGAUA--UGCCCugcaaaugcGUCCGGCGGCCc -3' miRNA: 3'- -UGUA---CUAUuuACGGG---------UAGGCCGCUGG- -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 54056 | 0.79 | 0.431231 |
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Target: 5'- gACAUcGGU-GAUGUUgAUCCGGCGACCa -3' miRNA: 3'- -UGUA-CUAuUUACGGgUAGGCCGCUGG- -5' |
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| 27291 | 3' | -50.6 | NC_005856.1 | + | 17891 | 1.13 | 0.003348 |
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Target: 5'- gACAUGAUAAAUGCCCAUCCGGCGACCu -3' miRNA: 3'- -UGUACUAUUUACGGGUAGGCCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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