Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27292 | 3' | -55.2 | NC_005856.1 | + | 59507 | 0.66 | 0.835598 |
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Target: 5'- cUGUCUCaggcgaagGUCACGCGuGCUCU-CCAg- -3' miRNA: 3'- -ACGGAGa-------CAGUGCGU-CGAGAaGGUgg -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 17070 | 0.66 | 0.833871 |
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Target: 5'- cGCCUCccggcgcagucgGUCAUaGCuuuuGC-CUUCCACCu -3' miRNA: 3'- aCGGAGa-----------CAGUG-CGu---CGaGAAGGUGG- -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 68610 | 0.66 | 0.818872 |
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Target: 5'- aGCCaCUGUCAUGauaaAGCUCUcugauguuuggcgcgCCACUg -3' miRNA: 3'- aCGGaGACAGUGCg---UCGAGAa--------------GGUGG- -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 28043 | 0.67 | 0.780594 |
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Target: 5'- aGCUg--GUUaaugGCGCAGCUCg-CCACCu -3' miRNA: 3'- aCGGagaCAG----UGCGUCGAGaaGGUGG- -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 25054 | 0.68 | 0.751005 |
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Target: 5'- --aUUCUGUCcaGCGCGGacuUCUUCCACUu -3' miRNA: 3'- acgGAGACAG--UGCGUCg--AGAAGGUGG- -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 27520 | 0.68 | 0.710007 |
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Target: 5'- cUGCCUCUuuaaugUugGCGGCUauccaugCCGCCg -3' miRNA: 3'- -ACGGAGAca----GugCGUCGAgaa----GGUGG- -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 22653 | 0.74 | 0.413402 |
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Target: 5'- aUGaCUUUGUCAUGCAGC---UCCACCg -3' miRNA: 3'- -ACgGAGACAGUGCGUCGagaAGGUGG- -5' |
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| 27292 | 3' | -55.2 | NC_005856.1 | + | 31975 | 1.13 | 0.00098 |
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Target: 5'- aUGCCUCUGUCACGCAGCUCUUCCACCa -3' miRNA: 3'- -ACGGAGACAGUGCGUCGAGAAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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