Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27293 | 3' | -54.9 | NC_005856.1 | + | 29183 | 0.66 | 0.85521 |
Target: 5'- -aGCGaCCUcg-CGGAUCAGgUGGCAGc -3' miRNA: 3'- gaCGC-GGGuaaGUCUGGUCaACCGUC- -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 69355 | 0.66 | 0.846918 |
Target: 5'- --aUGCCgGUUUAGcUCAGUUGGUAGa -3' miRNA: 3'- gacGCGGgUAAGUCuGGUCAACCGUC- -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 70477 | 0.66 | 0.845234 |
Target: 5'- uCUGUGCCUAUgcauugauauugCuGACCAGgaUGGCAu -3' miRNA: 3'- -GACGCGGGUAa-----------GuCUGGUCa-ACCGUc -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 9978 | 0.66 | 0.838412 |
Target: 5'- uCUGCGCaguaCCGUcCGGACgGGaaaUGGCGGa -3' miRNA: 3'- -GACGCG----GGUAaGUCUGgUCa--ACCGUC- -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 83500 | 0.67 | 0.811683 |
Target: 5'- uUGCGCCCAcUCaacucgacGGACCAauuccgGGCGGc -3' miRNA: 3'- gACGCGGGUaAG--------UCUGGUcaa---CCGUC- -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 14675 | 0.7 | 0.594869 |
Target: 5'- -cGCGCCCAUcuUCGGA-CAGUcGGCGc -3' miRNA: 3'- gaCGCGGGUA--AGUCUgGUCAaCCGUc -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 35996 | 1.09 | 0.00173 |
Target: 5'- cCUGCGCCCAUUCAGACCAGUUGGCAGa -3' miRNA: 3'- -GACGCGGGUAAGUCUGGUCAACCGUC- -5' |
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27293 | 3' | -54.9 | NC_005856.1 | + | 32735 | 1.09 | 0.00173 |
Target: 5'- cCUGCGCCCAUUCAGACCAGUUGGCAGa -3' miRNA: 3'- -GACGCGGGUAAGUCUGGUCAACCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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