Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27294 | 3' | -51 | NC_005856.1 | + | 35455 | 0.66 | 0.978357 |
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Target: 5'- gGGCGUGGCCUuaauguuucaggggaGACaCUuUCAGACCGu -3' miRNA: 3'- gCUGUAUUGGA---------------CUG-GAcAGUCUGGUc -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 48208 | 0.66 | 0.974705 |
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Target: 5'- uGACA--GCCUGAa-UGUUAGugCGGa -3' miRNA: 3'- gCUGUauUGGACUggACAGUCugGUC- -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 1276 | 0.66 | 0.971834 |
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Target: 5'- aGAgAU-ACCUGGCCUgGUCuGGACaCAGu -3' miRNA: 3'- gCUgUAuUGGACUGGA-CAG-UCUG-GUC- -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 36542 | 0.66 | 0.968736 |
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Target: 5'- aCGGCAauuuAUUUGcGCCUGUCgcaAGACCAGc -3' miRNA: 3'- -GCUGUau--UGGAC-UGGACAG---UCUGGUC- -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 40794 | 0.68 | 0.923899 |
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Target: 5'- uGACAUAccgauuUCUGGCUUGUCAGuaGCCGa -3' miRNA: 3'- gCUGUAUu-----GGACUGGACAGUC--UGGUc -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 22839 | 0.7 | 0.844094 |
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Target: 5'- gCGGCAUAACCUGAaUCUGaggCAGcACCuGg -3' miRNA: 3'- -GCUGUAUUGGACU-GGACa--GUC-UGGuC- -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 35985 | 0.72 | 0.769115 |
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Target: 5'- uGACGUauaGACCUGcGCCcauUCAGACCAGu -3' miRNA: 3'- gCUGUA---UUGGAC-UGGac-AGUCUGGUC- -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 32746 | 0.72 | 0.769115 |
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Target: 5'- uGACGUauaGACCUGcGCCcauUCAGACCAGu -3' miRNA: 3'- gCUGUA---UUGGAC-UGGac-AGUCUGGUC- -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 8183 | 0.73 | 0.6853 |
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Target: 5'- gGugGUGACCaGGCUguggGUCAGGCCAc -3' miRNA: 3'- gCugUAUUGGaCUGGa---CAGUCUGGUc -5' |
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| 27294 | 3' | -51 | NC_005856.1 | + | 33737 | 1.09 | 0.005058 |
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Target: 5'- aCGACAUAACCUGACCUGUCAGACCAGc -3' miRNA: 3'- -GCUGUAUUGGACUGGACAGUCUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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