miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27295 3' -54 NC_005856.1 + 87818 0.66 0.864907
Target:  5'- uUUGGUGGAucacucaugucaucAAUGAUGCCCGGa-GUGg -3'
miRNA:   3'- gAGCCGCCU--------------UUACUAUGGGUCggCAU- -5'
27295 3' -54 NC_005856.1 + 68824 0.67 0.832305
Target:  5'- aCUCGGUGGuuugucuggGAggacuagGCCCAGCCa-- -3'
miRNA:   3'- -GAGCCGCCuuua-----CUa------UGGGUCGGcau -5'
27295 3' -54 NC_005856.1 + 41287 0.67 0.825222
Target:  5'- cCUUGGCGGcuccGGUGCCaAGCCGa- -3'
miRNA:   3'- -GAGCCGCCuuuaCUAUGGgUCGGCau -5'
27295 3' -54 NC_005856.1 + 19217 0.69 0.748356
Target:  5'- -aUGGUGGAAGguc-GCCCGGUCGUAa -3'
miRNA:   3'- gaGCCGCCUUUacuaUGGGUCGGCAU- -5'
27295 3' -54 NC_005856.1 + 34280 1.07 0.003292
Target:  5'- uCUCGGCGGAAAUGAUACCCAGCCGUAg -3'
miRNA:   3'- -GAGCCGCCUUUACUAUGGGUCGGCAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.