miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27297 3' -55.4 NC_005856.1 + 36509 1.05 0.002741
Target:  5'- aAAAGAUCCCCCUGUUGAGCACGGCUAa -3'
miRNA:   3'- -UUUCUAGGGGGACAACUCGUGCCGAU- -5'
27297 3' -55.4 NC_005856.1 + 32222 1.05 0.002741
Target:  5'- aAAAGAUCCCCCUGUUGAGCACGGCUAa -3'
miRNA:   3'- -UUUCUAGGGGGACAACUCGUGCCGAU- -5'
27297 3' -55.4 NC_005856.1 + 37679 0.68 0.63885
Target:  5'- ---aAUCCCCCUgaauuuucgGUUuAGCGCGGCUGa -3'
miRNA:   3'- uuucUAGGGGGA---------CAAcUCGUGCCGAU- -5'
27297 3' -55.4 NC_005856.1 + 17360 0.67 0.745647
Target:  5'- cGGAGAacgggacaacaUCCUCCUGUccgcgcuggcUGuGCACGGCg- -3'
miRNA:   3'- -UUUCU-----------AGGGGGACA----------ACuCGUGCCGau -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.