Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27298 | 3' | -53.3 | NC_005856.1 | + | 21264 | 0.66 | 0.921879 |
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Target: 5'- -gCCCGUUugcuUGUCgCGCCAUGAUUCa -3' miRNA: 3'- agGGGUAGu---GCGGgGUGGUAUUAAGc -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 9917 | 0.66 | 0.921879 |
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Target: 5'- uUCCCCAggGCGUCCgugACCGggcAUUCGa -3' miRNA: 3'- -AGGGGUagUGCGGGg--UGGUau-UAAGC- -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 36331 | 0.67 | 0.89655 |
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Target: 5'- uUCCCUGUCAgCGCCCCggugaucccGCCGUugaaagUCu -3' miRNA: 3'- -AGGGGUAGU-GCGGGG---------UGGUAuua---AGc -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 32400 | 0.67 | 0.89655 |
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Target: 5'- uUCCCUGUCAgCGCCCCggugaucccGCCGUugaaagUCu -3' miRNA: 3'- -AGGGGUAGU-GCGGGG---------UGGUAuua---AGc -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 56954 | 0.67 | 0.882353 |
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Target: 5'- uUCCCUGUUauGCGCCCCGCacgGGggCGc -3' miRNA: 3'- -AGGGGUAG--UGCGGGGUGguaUUaaGC- -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 81020 | 0.75 | 0.437712 |
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Target: 5'- uUCCCCAUCACuGCCCUgaACCAgggcaucugcaaUAGUUCc -3' miRNA: 3'- -AGGGGUAGUG-CGGGG--UGGU------------AUUAAGc -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 48351 | 0.76 | 0.409915 |
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Target: 5'- aCCCUGUCACGUCCUGCCAccuGUUUGa -3' miRNA: 3'- aGGGGUAGUGCGGGGUGGUau-UAAGC- -5' |
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| 27298 | 3' | -53.3 | NC_005856.1 | + | 48407 | 1.1 | 0.002528 |
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Target: 5'- cUCCCCAUCACGCCCCACCAUAAUUCGg -3' miRNA: 3'- -AGGGGUAGUGCGGGGUGGUAUUAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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