miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27298 3' -53.3 NC_005856.1 + 21264 0.66 0.921879
Target:  5'- -gCCCGUUugcuUGUCgCGCCAUGAUUCa -3'
miRNA:   3'- agGGGUAGu---GCGGgGUGGUAUUAAGc -5'
27298 3' -53.3 NC_005856.1 + 9917 0.66 0.921879
Target:  5'- uUCCCCAggGCGUCCgugACCGggcAUUCGa -3'
miRNA:   3'- -AGGGGUagUGCGGGg--UGGUau-UAAGC- -5'
27298 3' -53.3 NC_005856.1 + 36331 0.67 0.89655
Target:  5'- uUCCCUGUCAgCGCCCCggugaucccGCCGUugaaagUCu -3'
miRNA:   3'- -AGGGGUAGU-GCGGGG---------UGGUAuua---AGc -5'
27298 3' -53.3 NC_005856.1 + 32400 0.67 0.89655
Target:  5'- uUCCCUGUCAgCGCCCCggugaucccGCCGUugaaagUCu -3'
miRNA:   3'- -AGGGGUAGU-GCGGGG---------UGGUAuua---AGc -5'
27298 3' -53.3 NC_005856.1 + 56954 0.67 0.882353
Target:  5'- uUCCCUGUUauGCGCCCCGCacgGGggCGc -3'
miRNA:   3'- -AGGGGUAG--UGCGGGGUGguaUUaaGC- -5'
27298 3' -53.3 NC_005856.1 + 81020 0.75 0.437712
Target:  5'- uUCCCCAUCACuGCCCUgaACCAgggcaucugcaaUAGUUCc -3'
miRNA:   3'- -AGGGGUAGUG-CGGGG--UGGU------------AUUAAGc -5'
27298 3' -53.3 NC_005856.1 + 48351 0.76 0.409915
Target:  5'- aCCCUGUCACGUCCUGCCAccuGUUUGa -3'
miRNA:   3'- aGGGGUAGUGCGGGGUGGUau-UAAGC- -5'
27298 3' -53.3 NC_005856.1 + 48407 1.1 0.002528
Target:  5'- cUCCCCAUCACGCCCCACCAUAAUUCGg -3'
miRNA:   3'- -AGGGGUAGUGCGGGGUGGUAUUAAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.