miRNA: 3'- -GGUCGUG----UGGUCgUAgUUCGUu-----AGCG- -5'
miRNA: 3'- -GGUCGUGUGgUCGUag-----UUCGUUAGCG- -5'
miRNA: 3'- -GGUCGUGUGGUCGUAGUUCGUUAGCG- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27301 | 3' | -50.6 | NC_005856.1 | + | 8060 | 0.75 | 0.608894 |
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Target: 5'- gCCAGCAUccggGCCAGgGUgGAGCAcccauuUCGCa -3' miRNA: 3'- -GGUCGUG----UGGUCgUAgUUCGUu-----AGCG- -5' |
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| 27301 | 3' | -50.6 | NC_005856.1 | + | 48804 | 0.75 | 0.597925 |
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Target: 5'- gUCGGCAUACaGGCAguaugcaAAGCGAUCGCu -3' miRNA: 3'- -GGUCGUGUGgUCGUag-----UUCGUUAGCG- -5' |
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| 27301 | 3' | -50.6 | NC_005856.1 | + | 50718 | 1.15 | 0.002164 |
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Target: 5'- aCCAGCACACCAGCAUCAAGCAAUCGCu -3' miRNA: 3'- -GGUCGUGUGGUCGUAGUUCGUUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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