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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27301 | 5' | -49.7 | NC_005856.1 | + | 63982 | 0.67 | 0.9774 |
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Target: 5'- gCugUGCUGGCCUuauUUGCUG---AUGCu -3' miRNA: 3'- gGugACGACUGGA---AACGGCcaaUAUG- -5' |
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| 27301 | 5' | -49.7 | NC_005856.1 | + | 86786 | 0.67 | 0.968648 |
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Target: 5'- -aACUGgaGGCUUaacUGCCGGUaGUGCc -3' miRNA: 3'- ggUGACgaCUGGAa--ACGGCCAaUAUG- -5' |
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| 27301 | 5' | -49.7 | NC_005856.1 | + | 88678 | 0.7 | 0.889657 |
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Target: 5'- gCUAUUGCUGugUUaUGCCgucaggaaauGGUUGUGCu -3' miRNA: 3'- -GGUGACGACugGAaACGG----------CCAAUAUG- -5' |
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| 27301 | 5' | -49.7 | NC_005856.1 | + | 64561 | 0.74 | 0.700915 |
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Target: 5'- gCAC-GCgGACCUguugGCUGGUUAUGCu -3' miRNA: 3'- gGUGaCGaCUGGAaa--CGGCCAAUAUG- -5' |
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| 27301 | 5' | -49.7 | NC_005856.1 | + | 49723 | 0.77 | 0.579672 |
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Target: 5'- aCCACUGCUGGCCUUUaCCcGUUAa-- -3' miRNA: 3'- -GGUGACGACUGGAAAcGGcCAAUaug -5' |
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| 27301 | 5' | -49.7 | NC_005856.1 | + | 50684 | 1.13 | 0.003501 |
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Target: 5'- gCCACUGCUGACCUUUGCCGGUUAUACg -3' miRNA: 3'- -GGUGACGACUGGAAACGGCCAAUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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