Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27302 | 3' | -40.7 | NC_005856.1 | + | 14860 | 0.66 | 1 |
Target: 5'- cGAUCcGCUugGGu-UGAAGGGAUuaaGCg -3' miRNA: 3'- aCUAGuUGGugCUuuACUUCUUUA---UG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 57885 | 0.99 | 0.23293 |
Target: 5'- cUGAUCAACCA-GAAAUGAAGAAAUACa -3' miRNA: 3'- -ACUAGUUGGUgCUUUACUUCUUUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 40415 | 0.73 | 0.999611 |
Target: 5'- aGAUCAccGCCAC-AAGUGAAGA--UGCc -3' miRNA: 3'- aCUAGU--UGGUGcUUUACUUCUuuAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 46308 | 0.72 | 0.999825 |
Target: 5'- gUGAUaugGAUCugGAuAAUGggGAAGUGCc -3' miRNA: 3'- -ACUAg--UUGGugCU-UUACuuCUUUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 50543 | 0.69 | 0.999996 |
Target: 5'- cGAUCAgaauuccguuAUCACGcaaccAGGUGAAGAGAUAg -3' miRNA: 3'- aCUAGU----------UGGUGC-----UUUACUUCUUUAUg -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 13246 | 0.68 | 1 |
Target: 5'- cUGAUC-GCCACGc--UGAAGGAGUu- -3' miRNA: 3'- -ACUAGuUGGUGCuuuACUUCUUUAug -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 45694 | 0.68 | 1 |
Target: 5'- gUGAUCAAUC-CGGAcgGuaccGGGAAUAUa -3' miRNA: 3'- -ACUAGUUGGuGCUUuaCu---UCUUUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 25842 | 0.67 | 1 |
Target: 5'- gUGAUCuGACUcuggACGAGAUGAA-AGAUGCc -3' miRNA: 3'- -ACUAG-UUGG----UGCUUUACUUcUUUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 57259 | 0.67 | 1 |
Target: 5'- cUGGUCAaccguACCACGcuGUGggGuucgGCu -3' miRNA: 3'- -ACUAGU-----UGGUGCuuUACuuCuuuaUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 59391 | 0.66 | 1 |
Target: 5'- -uGUUGGCgACGAAAUGggGAAc--- -3' miRNA: 3'- acUAGUUGgUGCUUUACuuCUUuaug -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 59919 | 0.66 | 1 |
Target: 5'- cUGGUCGccagGCCACauuuauUGAAGAGGUAa -3' miRNA: 3'- -ACUAGU----UGGUGcuuu--ACUUCUUUAUg -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 33489 | 0.66 | 1 |
Target: 5'- cGggCAGCUACccgcguucauauuGAAAUGAAGAauuaauguuGAUGCg -3' miRNA: 3'- aCuaGUUGGUG-------------CUUUACUUCU---------UUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 75181 | 0.66 | 1 |
Target: 5'- gUGGUCAAUaagCugGggGUGAAauuuGGUACa -3' miRNA: 3'- -ACUAGUUG---GugCuuUACUUcu--UUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 63501 | 0.7 | 0.999991 |
Target: 5'- gGAgCAACCGCuGAGUGucagcAGGAAUACg -3' miRNA: 3'- aCUaGUUGGUGcUUUACu----UCUUUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 91037 | 0.66 | 1 |
Target: 5'- cGAUCAGCaaaaagACGAuGAUGAAGG--UACu -3' miRNA: 3'- aCUAGUUGg-----UGCU-UUACUUCUuuAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 27914 | 0.66 | 1 |
Target: 5'- aGAUCAGCCA---GGUGgcGAGcUGCg -3' miRNA: 3'- aCUAGUUGGUgcuUUACuuCUUuAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 57155 | 0.67 | 1 |
Target: 5'- cGGUUGACCAgcgcCGGAGUGGucGGAUGCu -3' miRNA: 3'- aCUAGUUGGU----GCUUUACUucUUUAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 71297 | 0.67 | 1 |
Target: 5'- uUGAUaacuGCCGCuGGGAUGAAGu--UGCa -3' miRNA: 3'- -ACUAgu--UGGUG-CUUUACUUCuuuAUG- -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 25696 | 0.68 | 1 |
Target: 5'- uUGAUCGGCCcACuGAAUGGAGGc---- -3' miRNA: 3'- -ACUAGUUGG-UGcUUUACUUCUuuaug -5' |
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27302 | 3' | -40.7 | NC_005856.1 | + | 56496 | 0.68 | 1 |
Target: 5'- aUGGUUAACuCACGGGuaGguGAAAUACa -3' miRNA: 3'- -ACUAGUUG-GUGCUUuaCuuCUUUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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