Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27303 | 3' | -45.6 | NC_005856.1 | + | 78397 | 0.66 | 0.999775 |
Target: 5'- cGAUCCAAUAcUCAGG-----GCAGAg -3' miRNA: 3'- uCUAGGUUAUuAGUCCagguaUGUCU- -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 32623 | 0.66 | 0.999627 |
Target: 5'- uGGUCUgAAUGGgcgCAGGUCUAUACGu- -3' miRNA: 3'- uCUAGG-UUAUUa--GUCCAGGUAUGUcu -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 36108 | 0.66 | 0.999627 |
Target: 5'- uGGUCUgAAUGGgcgCAGGUCUAUACGu- -3' miRNA: 3'- uCUAGG-UUAUUa--GUCCAGGUAUGUcu -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 73221 | 0.67 | 0.999399 |
Target: 5'- uGAUCCGGggaauauuUCGGuaauUCCAUACAGAc -3' miRNA: 3'- uCUAGGUUauu-----AGUCc---AGGUAUGUCU- -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 11599 | 0.67 | 0.999246 |
Target: 5'- cGAUCCgGGUAAaccgcaugcuUCaguGGGUCCGUAUAGGu -3' miRNA: 3'- uCUAGG-UUAUU----------AG---UCCAGGUAUGUCU- -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 28867 | 0.7 | 0.988034 |
Target: 5'- cGAUCCGgauggcagcguguacAUGAUCaAGGcuaaaUCCAUACAGGg -3' miRNA: 3'- uCUAGGU---------------UAUUAG-UCC-----AGGUAUGUCU- -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 13407 | 0.76 | 0.86581 |
Target: 5'- uGAUCCGgcgAUAGUaCAGGUCCAUGuCAGu -3' miRNA: 3'- uCUAGGU---UAUUA-GUCCAGGUAU-GUCu -5' |
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27303 | 3' | -45.6 | NC_005856.1 | + | 62918 | 1.1 | 0.014635 |
Target: 5'- uAGAUCCAAUAAUCAGGUCCAUACAGAu -3' miRNA: 3'- -UCUAGGUUAUUAGUCCAGGUAUGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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