Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27304 | 3' | -52.2 | NC_005856.1 | + | 8332 | 0.66 | 0.947479 |
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Target: 5'- aGGCCcACAUUGGugUCgauGCCaagaguGGCCUGa -3' miRNA: 3'- aUUGGcUGUAACUugAG---UGG------CCGGAC- -5' |
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| 27304 | 3' | -52.2 | NC_005856.1 | + | 71967 | 0.67 | 0.921093 |
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Target: 5'- cAGCUGAUgaUGcGCUCACUGGCUUc -3' miRNA: 3'- aUUGGCUGuaACuUGAGUGGCCGGAc -5' |
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| 27304 | 3' | -52.2 | NC_005856.1 | + | 57684 | 0.68 | 0.880514 |
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Target: 5'- cGACCGACAccuUUGAcaucGCUCuGCCGGUUa- -3' miRNA: 3'- aUUGGCUGU---AACU----UGAG-UGGCCGGac -5' |
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| 27304 | 3' | -52.2 | NC_005856.1 | + | 14775 | 0.68 | 0.848372 |
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Target: 5'- gGGCgCGGCAUccUGGGCUucggCACCGGgCUGg -3' miRNA: 3'- aUUG-GCUGUA--ACUUGA----GUGGCCgGAC- -5' |
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| 27304 | 3' | -52.2 | NC_005856.1 | + | 55954 | 0.7 | 0.793636 |
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Target: 5'- gUGGuuGGCAUU--ACUCACCGGCUg- -3' miRNA: 3'- -AUUggCUGUAAcuUGAGUGGCCGGac -5' |
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| 27304 | 3' | -52.2 | NC_005856.1 | + | 68291 | 1.08 | 0.004118 |
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Target: 5'- gUAACCGACAUUGAACUCACCGGCCUGg -3' miRNA: 3'- -AUUGGCUGUAACUUGAGUGGCCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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