miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27307 3' -52.1 NC_005856.1 + 8170 0.66 0.937233
Target:  5'- gCUCGUUgGCCGcGGuGguGAccaGGCUGUGg -3'
miRNA:   3'- gGAGUAA-CGGCaCC-CguCU---UCGAUAU- -5'
27307 3' -52.1 NC_005856.1 + 74245 1.09 0.00342
Target:  5'- gCCUCAUUGCCGUGGGCAGAAGCUAUAu -3'
miRNA:   3'- -GGAGUAACGGCACCCGUCUUCGAUAU- -5'
27307 3' -52.1 NC_005856.1 + 76977 0.66 0.920414
Target:  5'- uCCUguUUGCCGUGGuGCG--AGCUu-- -3'
miRNA:   3'- -GGAguAACGGCACC-CGUcuUCGAuau -5'
27307 3' -52.1 NC_005856.1 + 85422 0.69 0.829352
Target:  5'- gCUUUAUUGCCGUuGGUGGAuGCUGg- -3'
miRNA:   3'- -GGAGUAACGGCAcCCGUCUuCGAUau -5'
27307 3' -52.1 NC_005856.1 + 89562 0.67 0.907825
Target:  5'- uCC-CGUccaUGCUGUGGGUGuGGAGCUGc- -3'
miRNA:   3'- -GGaGUA---ACGGCACCCGU-CUUCGAUau -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.