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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 27311 | 3' | -49.1 | NC_005856.1 | + | 27461 | 0.65 | 0.991583 |
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Target: 5'- uUCUAUGcUGGUGGCCAuuACcaccugaucgcuuaAGCUCauCCGu -3' miRNA: 3'- -AGAUAUuACCACCGGU--UG--------------UUGAG--GGU- -5' |
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| 27311 | 3' | -49.1 | NC_005856.1 | + | 71275 | 0.66 | 0.986002 |
|
Target: 5'- gCUGUAcuUGGUGGCUuaaugcuuGAUAACUgCCGc -3' miRNA: 3'- aGAUAUu-ACCACCGG--------UUGUUGAgGGU- -5' |
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| 27311 | 3' | -49.1 | NC_005856.1 | + | 49347 | 0.67 | 0.981989 |
|
Target: 5'- ------cUGGUGGCCG-CAGC-CCCu -3' miRNA: 3'- agauauuACCACCGGUuGUUGaGGGu -5' |
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| 27311 | 3' | -49.1 | NC_005856.1 | + | 28278 | 0.68 | 0.971462 |
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Target: 5'- gUCUgGUGAguUGGUGGCCA---GCUCCa- -3' miRNA: 3'- -AGA-UAUU--ACCACCGGUuguUGAGGgu -5' |
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| 27311 | 3' | -49.1 | NC_005856.1 | + | 94158 | 1.1 | 0.005776 |
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Target: 5'- uUCUAUAAUGGUGGCCAACAACUCCCAg -3' miRNA: 3'- -AGAUAUUACCACCGGUUGUUGAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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