Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27314 | 3' | -49.2 | NC_005856.1 | + | 2443 | 0.7 | 0.921241 |
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Target: 5'- cCAag-AGCAuugGCGAuGCGCCGuuCCUCCg -3' miRNA: 3'- aGUaaaUCGU---UGUUuCGCGGC--GGAGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 40837 | 0.7 | 0.915037 |
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Target: 5'- -uGUUUAGCcACucauauaaacGAGCGCCccagGCCUCCu -3' miRNA: 3'- agUAAAUCGuUGu---------UUCGCGG----CGGAGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 87343 | 0.71 | 0.894746 |
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Target: 5'- ---aUUGGCAACGAGGUuaCGCUgaUCCg -3' miRNA: 3'- aguaAAUCGUUGUUUCGcgGCGG--AGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 50238 | 0.72 | 0.829138 |
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Target: 5'- aCAgcgAGCAAUucaugggcaAAAGUGCCGCCgCCa -3' miRNA: 3'- aGUaaaUCGUUG---------UUUCGCGGCGGaGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 83063 | 0.73 | 0.770827 |
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Target: 5'- ----aUGGCAuuACuGAGCGCCGUUUCCc -3' miRNA: 3'- aguaaAUCGU--UGuUUCGCGGCGGAGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 28813 | 0.74 | 0.760529 |
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Target: 5'- uUCAUUgauGCAccauuACuGAGCGUCGCUUCCc -3' miRNA: 3'- -AGUAAau-CGU-----UGuUUCGCGGCGGAGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 14158 | 0.74 | 0.760529 |
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Target: 5'- -----cGGCGAC---GCGCCGCCUCUu -3' miRNA: 3'- aguaaaUCGUUGuuuCGCGGCGGAGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 54726 | 0.74 | 0.759492 |
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Target: 5'- gCAUUUcaucagaagugccAGCAACAAcaaaCGCCGCUUCCa -3' miRNA: 3'- aGUAAA-------------UCGUUGUUuc--GCGGCGGAGG- -5' |
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| 27314 | 3' | -49.2 | NC_005856.1 | + | 9606 | 1.13 | 0.004002 |
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Target: 5'- aUCAUUUAGCAACAAAGCGCCGCCUCCc -3' miRNA: 3'- -AGUAAAUCGUUGUUUCGCGGCGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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