Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
27316 | 3' | -51.5 | NC_005856.1 | + | 22177 | 0.66 | 0.960685 |
Target: 5'- ---cAGCACAAUGGgggUAACCGCGAguUUCa -3' miRNA: 3'- guucUCGUGUUGCU---GUUGGCGCU--GAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 36053 | 0.66 | 0.956778 |
Target: 5'- --cGGGCGCAugGGC-ACCGCucgugccACUCc -3' miRNA: 3'- guuCUCGUGUugCUGuUGGCGc------UGAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 32678 | 0.66 | 0.956778 |
Target: 5'- --cGGGCGCAugGGC-ACCGCucgugccACUCc -3' miRNA: 3'- guuCUCGUGUugCUGuUGGCGc------UGAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 93845 | 0.67 | 0.933312 |
Target: 5'- ---cGGCAgAACGACAACCG-GGCg- -3' miRNA: 3'- guucUCGUgUUGCUGUUGGCgCUGag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 84293 | 0.67 | 0.924379 |
Target: 5'- aCAGGAGCugAaaauaugaacACGGCAuucaaaaucauuaugGCCGCGAUc- -3' miRNA: 3'- -GUUCUCGugU----------UGCUGU---------------UGGCGCUGag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 17900 | 0.68 | 0.915991 |
Target: 5'- --cGAGguCAGCGGCAACCuggcgggauuuaGCGGCg- -3' miRNA: 3'- guuCUCguGUUGCUGUUGG------------CGCUGag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 54889 | 0.68 | 0.909673 |
Target: 5'- ---uAGUuuagaAGCGACGACCGCGACg- -3' miRNA: 3'- guucUCGug---UUGCUGUUGGCGCUGag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 7308 | 0.68 | 0.889117 |
Target: 5'- ---aAGCACGGCGACAACUGa-GCUUa -3' miRNA: 3'- guucUCGUGUUGCUGUUGGCgcUGAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 77726 | 0.69 | 0.874117 |
Target: 5'- uGAuGGCACAGgGAUcaaAGCCGCGACa- -3' miRNA: 3'- gUUcUCGUGUUgCUG---UUGGCGCUGag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 41731 | 0.69 | 0.866244 |
Target: 5'- --cGAGCACGAUGAguauugaccuUGAUCGCGuACUCa -3' miRNA: 3'- guuCUCGUGUUGCU----------GUUGGCGC-UGAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 31107 | 0.69 | 0.848936 |
Target: 5'- gCAGGAGCGCAAUaGCAACUggagcuaauuccuGUGugUCa -3' miRNA: 3'- -GUUCUCGUGUUGcUGUUGG-------------CGCugAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 15011 | 0.7 | 0.840342 |
Target: 5'- aAAGAuaGCGCGACGGCGaacggcuACCGCGAg-- -3' miRNA: 3'- gUUCU--CGUGUUGCUGU-------UGGCGCUgag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 66954 | 0.71 | 0.765636 |
Target: 5'- gGAGuGGCACAACGACGccgaCGCGugUUu -3' miRNA: 3'- gUUC-UCGUGUUGCUGUug--GCGCugAG- -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 16922 | 0.73 | 0.67059 |
Target: 5'- ---uGGUACggUGAUGACCGCGACUg -3' miRNA: 3'- guucUCGUGuuGCUGUUGGCGCUGAg -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 20639 | 0.74 | 0.616016 |
Target: 5'- ---cAGCGCAAUGACAAgCGCGGCa- -3' miRNA: 3'- guucUCGUGUUGCUGUUgGCGCUGag -5' |
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27316 | 3' | -51.5 | NC_005856.1 | + | 18712 | 1.11 | 0.003183 |
Target: 5'- aCAAGAGCACAACGACAACCGCGACUCg -3' miRNA: 3'- -GUUCUCGUGUUGCUGUUGGCGCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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