miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27317 3' -48.2 NC_005856.1 + 87132 0.66 0.995947
Target:  5'- aCCGCC-AUUUauacuggcGAGGAUGccGCAGCGACc -3'
miRNA:   3'- -GGUGGcUAAAa-------CUCUUGC--UGUCGCUG- -5'
27317 3' -48.2 NC_005856.1 + 13435 0.66 0.995947
Target:  5'- gCCACCagcgaaGAcGACGACAGUGAUg -3'
miRNA:   3'- -GGUGGcuaaaaCUcUUGCUGUCGCUG- -5'
27317 3' -48.2 NC_005856.1 + 40081 0.66 0.995947
Target:  5'- aCCAuCCGAgcagUUGGGGguuCGAUucGGCGAUg -3'
miRNA:   3'- -GGU-GGCUaa--AACUCUu--GCUG--UCGCUG- -5'
27317 3' -48.2 NC_005856.1 + 80433 0.67 0.995229
Target:  5'- uUugCGAagaUGAGAAUGAaauugcCAGCGGCa -3'
miRNA:   3'- gGugGCUaaaACUCUUGCU------GUCGCUG- -5'
27317 3' -48.2 NC_005856.1 + 75467 0.67 0.991262
Target:  5'- aCCGCCGAcaUUGGcAAUGugAGUGAa -3'
miRNA:   3'- -GGUGGCUaaAACUcUUGCugUCGCUg -5'
27317 3' -48.2 NC_005856.1 + 54627 0.67 0.995229
Target:  5'- gCGCCGAUUUUu-GAGCcGCAGUcaGACg -3'
miRNA:   3'- gGUGGCUAAAAcuCUUGcUGUCG--CUG- -5'
27317 3' -48.2 NC_005856.1 + 78231 0.68 0.989946
Target:  5'- gCCGCCGGgcUUGAGGAUGAgucuCAGgguaUGACu -3'
miRNA:   3'- -GGUGGCUaaAACUCUUGCU----GUC----GCUG- -5'
27317 3' -48.2 NC_005856.1 + 57857 0.7 0.951222
Target:  5'- uCCGgCGcUUUgGAGAACGACAG-GGCa -3'
miRNA:   3'- -GGUgGCuAAAaCUCUUGCUGUCgCUG- -5'
27317 3' -48.2 NC_005856.1 + 61874 0.71 0.925667
Target:  5'- gCUACgGAUUcgcGcGAACGAUAGCGGCa -3'
miRNA:   3'- -GGUGgCUAAaa-CuCUUGCUGUCGCUG- -5'
27317 3' -48.2 NC_005856.1 + 30645 0.72 0.908982
Target:  5'- cCCACUGAauauauggguaaagGAGAGCGACuGUGGCa -3'
miRNA:   3'- -GGUGGCUaaaa----------CUCUUGCUGuCGCUG- -5'
27317 3' -48.2 NC_005856.1 + 22671 1.13 0.006761
Target:  5'- uCCACCGAUUUUGAGAACGACAGCGACu -3'
miRNA:   3'- -GGUGGCUAAAACUCUUGCUGUCGCUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.