Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27318 | 3' | -50.5 | NC_005856.1 | + | 72967 | 0.66 | 0.971796 |
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Target: 5'- gAUACCUGGCGGuaccuGGAUaAUCCcgGUc-- -3' miRNA: 3'- -UGUGGACCGCUu----CCUA-UAGGa-CAuug -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 14446 | 0.67 | 0.961616 |
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Target: 5'- gGCACCUGGCGAAucuGAUcgCCa----- -3' miRNA: 3'- -UGUGGACCGCUUc--CUAuaGGacauug -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 72050 | 0.67 | 0.957719 |
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Target: 5'- gGCGCggguuuCUGGCGcaauGGGUAUUCUGUugGACa -3' miRNA: 3'- -UGUG------GACCGCuu--CCUAUAGGACA--UUG- -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 10433 | 0.68 | 0.934211 |
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Target: 5'- gACGCCcGGCGAaccGGGcgcUCCUGUuauGCg -3' miRNA: 3'- -UGUGGaCCGCU---UCCuauAGGACAu--UG- -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 32523 | 0.69 | 0.910411 |
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Target: 5'- cGCugCUGGCGAAGGugaguUAUUCauUGGCu -3' miRNA: 3'- -UGugGACCGCUUCCu----AUAGGacAUUG- -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 36208 | 0.69 | 0.910411 |
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Target: 5'- cGCugCUGGCGAAGGugaguUAUUCauUGGCu -3' miRNA: 3'- -UGugGACCGCUUCCu----AUAGGacAUUG- -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 37738 | 0.69 | 0.903765 |
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Target: 5'- -aGCCUGGCGGuGGGAUGaaCCUGUu-- -3' miRNA: 3'- ugUGGACCGCU-UCCUAUa-GGACAuug -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 28715 | 0.69 | 0.896846 |
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Target: 5'- -aACCUGaaucaGCGGAGGAUA-CCgGUAGCu -3' miRNA: 3'- ugUGGAC-----CGCUUCCUAUaGGaCAUUG- -5' |
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| 27318 | 3' | -50.5 | NC_005856.1 | + | 26233 | 1.11 | 0.003831 |
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Target: 5'- gACACCUGGCGAAGGAUAUCCUGUAACg -3' miRNA: 3'- -UGUGGACCGCUUCCUAUAGGACAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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