miRNA: 3'- gUUAGUGACAGC---------AGCCGaa--GAC---UUGU- -5'
miRNA: 3'- guUAGUGACAG-CAGC-CGAaGACUUGu -5'
miRNA: 3'- gUUAGUGACAGCag------CCgaAGACUUGU- -5'
miRNA: 3'- guUAGUGACAGCAGCcGAAG--ACUugU- -5'
miRNA: 3'- -GUUAGUGACAGCAGCCGAAGACUUGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27319 | 5' | -49 | NC_005856.1 | + | 22793 | 0.66 | 0.991074 |
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Target: 5'- aAGUCGCUGUCGuucucaaaaUCGGUggagCUGcauGACAa -3' miRNA: 3'- gUUAGUGACAGC---------AGCCGaa--GAC---UUGU- -5' |
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| 27319 | 5' | -49 | NC_005856.1 | + | 37111 | 0.67 | 0.984724 |
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Target: 5'- -uGUCGCUGUUaGUUGcGCUaCUGAGCu -3' miRNA: 3'- guUAGUGACAG-CAGC-CGAaGACUUGu -5' |
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| 27319 | 5' | -49 | NC_005856.1 | + | 63309 | 0.67 | 0.980478 |
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Target: 5'- aAAUCACUGUUGgcaagccaGGa-UCUGAACAa -3' miRNA: 3'- gUUAGUGACAGCag------CCgaAGACUUGU- -5' |
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| 27319 | 5' | -49 | NC_005856.1 | + | 13314 | 0.7 | 0.918039 |
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Target: 5'- -cAUCACUGUCGUCGuCUUCgcUGGugGc -3' miRNA: 3'- guUAGUGACAGCAGCcGAAG--ACUugU- -5' |
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| 27319 | 5' | -49 | NC_005856.1 | + | 30613 | 1.09 | 0.008237 |
|
Target: 5'- uCAAUCACUGUCGUCGGCUUCUGAACAu -3' miRNA: 3'- -GUUAGUGACAGCAGCCGAAGACUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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