Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27321 | 3' | -49.4 | NC_005856.1 | + | 31101 | 1.1 | 0.004896 |
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Target: 5'- cUGAAAGCAGGAGCGCAAUAGCAACUGg -3' miRNA: 3'- -ACUUUCGUCCUCGCGUUAUCGUUGAC- -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 85071 | 0.7 | 0.90633 |
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Target: 5'- aGAAAGCuGGAGUGCuaaucccuggguuacGAggcUGGCAACUa -3' miRNA: 3'- aCUUUCGuCCUCGCG---------------UU---AUCGUUGAc -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 71616 | 0.69 | 0.92305 |
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Target: 5'- gGAuGGCAGGGGCaGCAAUGaauuGCUGg -3' miRNA: 3'- aCUuUCGUCCUCG-CGUUAUcgu-UGAC- -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 28416 | 0.67 | 0.972513 |
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Target: 5'- aGAAAGCGGGGuauCGUaAGUAGCAAUg- -3' miRNA: 3'- aCUUUCGUCCUc--GCG-UUAUCGUUGac -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 25478 | 0.66 | 0.984852 |
|
Target: 5'- -uGAAGCAucGGAGgGCAuggGGguACUGa -3' miRNA: 3'- acUUUCGU--CCUCgCGUua-UCguUGAC- -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 17736 | 0.66 | 0.978105 |
|
Target: 5'- aGAGAGCGcgugcguaauauGGAuguucaGCGCGGUGGCGaucguGCUGu -3' miRNA: 3'- aCUUUCGU------------CCU------CGCGUUAUCGU-----UGAC- -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 27992 | 0.67 | 0.975425 |
|
Target: 5'- aGAAAGCGGGGGU-----AGCAGCa- -3' miRNA: 3'- aCUUUCGUCCUCGcguuaUCGUUGac -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 73808 | 0.67 | 0.972513 |
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Target: 5'- cUGAAGGCGGGGGgaaGU-AUGGCGAUa- -3' miRNA: 3'- -ACUUUCGUCCUCg--CGuUAUCGUUGac -5' |
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| 27321 | 3' | -49.4 | NC_005856.1 | + | 41562 | 0.68 | 0.939906 |
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Target: 5'- cGAGGaaAGGGGacaGCAAcGGCAGCUGg -3' miRNA: 3'- aCUUUcgUCCUCg--CGUUaUCGUUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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