Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27323 | 3' | -50.1 | NC_005856.1 | + | 15591 | 0.66 | 0.97163 |
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Target: 5'- aGCUCGUCAGaaAUAGugccAUCCAGCUu- -3' miRNA: 3'- -CGAGCAGUCccUAUUu---UGGGUCGAua -5' |
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| 27323 | 3' | -50.1 | NC_005856.1 | + | 66557 | 0.67 | 0.968451 |
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Target: 5'- uGCUCGUCuGGGGUAc-GCCUugucaGGCa-- -3' miRNA: 3'- -CGAGCAGuCCCUAUuuUGGG-----UCGaua -5' |
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| 27323 | 3' | -50.1 | NC_005856.1 | + | 67612 | 0.69 | 0.895904 |
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Target: 5'- aUUCGaCAGGGGUAAGACggcggaCAGCUAc -3' miRNA: 3'- cGAGCaGUCCCUAUUUUGg-----GUCGAUa -5' |
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| 27323 | 3' | -50.1 | NC_005856.1 | + | 43988 | 0.7 | 0.88864 |
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Target: 5'- ---aGUCAGGGAgUAAGGCCgCAGCg-- -3' miRNA: 3'- cgagCAGUCCCU-AUUUUGG-GUCGaua -5' |
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| 27323 | 3' | -50.1 | NC_005856.1 | + | 68817 | 0.71 | 0.83067 |
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Target: 5'- gGUUUGUCuGGGAggacuAGGCCCAGCc-- -3' miRNA: 3'- -CGAGCAGuCCCUau---UUUGGGUCGaua -5' |
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| 27323 | 3' | -50.1 | NC_005856.1 | + | 34121 | 1.09 | 0.005303 |
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Target: 5'- aGCUCGUCAGGGAUAAAACCCAGCUAUg -3' miRNA: 3'- -CGAGCAGUCCCUAUUUUGGGUCGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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