miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27327 3' -50.3 NC_005856.1 + 28093 0.66 0.97238
Target:  5'- gGAAGCGAUUGCa--GCccuucagACUGCCg- -3'
miRNA:   3'- -CUUCGCUAGCGaaaCGua-----UGACGGac -5'
27327 3' -50.3 NC_005856.1 + 21473 0.66 0.965872
Target:  5'- -cGGCGAgCGCcgaucaUUUGCAUGgUGCCg- -3'
miRNA:   3'- cuUCGCUaGCG------AAACGUAUgACGGac -5'
27327 3' -50.3 NC_005856.1 + 12081 0.67 0.94977
Target:  5'- -cGGCGAUCGCUcaugGCGUGuagGCCa- -3'
miRNA:   3'- cuUCGCUAGCGAaa--CGUAUga-CGGac -5'
27327 3' -50.3 NC_005856.1 + 2242 0.7 0.841126
Target:  5'- cGAGCGAUCGCUc-GUucucugGCUGCCUu -3'
miRNA:   3'- cUUCGCUAGCGAaaCGua----UGACGGAc -5'
27327 3' -50.3 NC_005856.1 + 11207 0.71 0.803902
Target:  5'- cGAuGCGcgCGCUUgcUGCGUccAUUGCCUGc -3'
miRNA:   3'- -CUuCGCuaGCGAA--ACGUA--UGACGGAC- -5'
27327 3' -50.3 NC_005856.1 + 50594 0.74 0.655182
Target:  5'- uGAAGCGAUUGCUUgaugcugGUGUGCUGguaCCUGu -3'
miRNA:   3'- -CUUCGCUAGCGAAa------CGUAUGAC---GGAC- -5'
27327 3' -50.3 NC_005856.1 + 48675 1.09 0.005058
Target:  5'- cGAAGCGAUCGCUUUGCAUACUGCCUGu -3'
miRNA:   3'- -CUUCGCUAGCGAAACGUAUGACGGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.