Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27327 | 3' | -50.3 | NC_005856.1 | + | 28093 | 0.66 | 0.97238 |
Target: 5'- gGAAGCGAUUGCa--GCccuucagACUGCCg- -3' miRNA: 3'- -CUUCGCUAGCGaaaCGua-----UGACGGac -5' |
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27327 | 3' | -50.3 | NC_005856.1 | + | 21473 | 0.66 | 0.965872 |
Target: 5'- -cGGCGAgCGCcgaucaUUUGCAUGgUGCCg- -3' miRNA: 3'- cuUCGCUaGCG------AAACGUAUgACGGac -5' |
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27327 | 3' | -50.3 | NC_005856.1 | + | 12081 | 0.67 | 0.94977 |
Target: 5'- -cGGCGAUCGCUcaugGCGUGuagGCCa- -3' miRNA: 3'- cuUCGCUAGCGAaa--CGUAUga-CGGac -5' |
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27327 | 3' | -50.3 | NC_005856.1 | + | 2242 | 0.7 | 0.841126 |
Target: 5'- cGAGCGAUCGCUc-GUucucugGCUGCCUu -3' miRNA: 3'- cUUCGCUAGCGAaaCGua----UGACGGAc -5' |
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27327 | 3' | -50.3 | NC_005856.1 | + | 11207 | 0.71 | 0.803902 |
Target: 5'- cGAuGCGcgCGCUUgcUGCGUccAUUGCCUGc -3' miRNA: 3'- -CUuCGCuaGCGAA--ACGUA--UGACGGAC- -5' |
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27327 | 3' | -50.3 | NC_005856.1 | + | 50594 | 0.74 | 0.655182 |
Target: 5'- uGAAGCGAUUGCUUgaugcugGUGUGCUGguaCCUGu -3' miRNA: 3'- -CUUCGCUAGCGAAa------CGUAUGAC---GGAC- -5' |
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27327 | 3' | -50.3 | NC_005856.1 | + | 48675 | 1.09 | 0.005058 |
Target: 5'- cGAAGCGAUCGCUUUGCAUACUGCCUGu -3' miRNA: 3'- -CUUCGCUAGCGAAACGUAUGACGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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