Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27328 | 5' | -57.6 | NC_005856.1 | + | 2230 | 0.66 | 0.723907 |
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Target: 5'- cGUUCUCUGGCUgCCUUCU---GCCAc -3' miRNA: 3'- uCAAGGGGCCGGaGGAGGAaauCGGU- -5' |
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| 27328 | 5' | -57.6 | NC_005856.1 | + | 36195 | 0.67 | 0.640658 |
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Target: 5'- uGUUCCCUGuGCauucgCCUCCag-GGCCGu -3' miRNA: 3'- uCAAGGGGC-CGga---GGAGGaaaUCGGU- -5' |
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| 27328 | 5' | -57.6 | NC_005856.1 | + | 32536 | 0.67 | 0.640658 |
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Target: 5'- uGUUCCCUGuGCauucgCCUCCag-GGCCGu -3' miRNA: 3'- uCAAGGGGC-CGga---GGAGGaaaUCGGU- -5' |
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| 27328 | 5' | -57.6 | NC_005856.1 | + | 41042 | 0.71 | 0.4293 |
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Target: 5'- cAGUUCCCUGGCCUuucgCCgagUCUUUGGUg- -3' miRNA: 3'- -UCAAGGGGCCGGA----GGa--GGAAAUCGgu -5' |
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| 27328 | 5' | -57.6 | NC_005856.1 | + | 40858 | 0.75 | 0.24823 |
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Target: 5'- gAGcgCCCCaGGCCUCCUCCUgggacucauacguuuUUAcGCCGg -3' miRNA: 3'- -UCaaGGGG-CCGGAGGAGGA---------------AAU-CGGU- -5' |
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| 27328 | 5' | -57.6 | NC_005856.1 | + | 54518 | 1.09 | 0.001121 |
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Target: 5'- gAGUUCCCCGGCCUCCUCCUUUAGCCAa -3' miRNA: 3'- -UCAAGGGGCCGGAGGAGGAAAUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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