Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2733 | 3' | -56.3 | NC_001491.2 | + | 67012 | 0.66 | 0.905243 |
Target: 5'- cCGCaaCGGgugugUGGUUGGGGAGGUGu-- -3' miRNA: 3'- aGCGg-GCUa----ACCAGCCCCUUCACuca -5' |
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2733 | 3' | -56.3 | NC_001491.2 | + | 123707 | 0.66 | 0.881519 |
Target: 5'- aCGCCCGcucgauaguugagguAUUGGUgGGGGAAaUGGa- -3' miRNA: 3'- aGCGGGC---------------UAACCAgCCCCUUcACUca -5' |
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2733 | 3' | -56.3 | NC_001491.2 | + | 137512 | 0.69 | 0.751637 |
Target: 5'- aCGUCCGGUagcUGGUCGaccaGGGggGcGAGg -3' miRNA: 3'- aGCGGGCUA---ACCAGC----CCCuuCaCUCa -5' |
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2733 | 3' | -56.3 | NC_001491.2 | + | 112829 | 0.69 | 0.732294 |
Target: 5'- -gGCCgGAgaUGGUUGGGGAGG-GGGa -3' miRNA: 3'- agCGGgCUa-ACCAGCCCCUUCaCUCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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