Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27333 | 5' | -59 | NC_005857.1 | + | 51472 | 0.66 | 0.449987 |
Target: 5'- cUCGACugAGGCAGCUGCuGCcAgGGGgUa -3' miRNA: 3'- aAGCUG--UCCGUCGACG-CGcUgCCUgA- -5' |
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27333 | 5' | -59 | NC_005857.1 | + | 4595 | 0.69 | 0.317931 |
Target: 5'- ---uGCAGGCAGC--CGCGAUGGACc -3' miRNA: 3'- aagcUGUCCGUCGacGCGCUGCCUGa -5' |
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27333 | 5' | -59 | NC_005857.1 | + | 47786 | 0.7 | 0.253791 |
Target: 5'- -aCGGCAcGCAGCUGCGCcACGG-Ca -3' miRNA: 3'- aaGCUGUcCGUCGACGCGcUGCCuGa -5' |
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27333 | 5' | -59 | NC_005857.1 | + | 13929 | 0.71 | 0.217213 |
Target: 5'- cUCGAUgagGGGCAGCUcuccGCGCgGAUGGAUg -3' miRNA: 3'- aAGCUG---UCCGUCGA----CGCG-CUGCCUGa -5' |
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27333 | 5' | -59 | NC_005857.1 | + | 37592 | 0.74 | 0.141139 |
Target: 5'- cUCGGCuGGCAGCguaucgaccaGCGCGGCGGGa- -3' miRNA: 3'- aAGCUGuCCGUCGa---------CGCGCUGCCUga -5' |
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27333 | 5' | -59 | NC_005857.1 | + | 20838 | 0.74 | 0.133558 |
Target: 5'- -gCGACGGGCAGCgGCaCGcCGGGCUa -3' miRNA: 3'- aaGCUGUCCGUCGaCGcGCuGCCUGA- -5' |
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27333 | 5' | -59 | NC_005857.1 | + | 2661 | 1.04 | 0.000719 |
Target: 5'- gUUCGACAGGCAGCUGCGCGACGGACUc -3' miRNA: 3'- -AAGCUGUCCGUCGACGCGCUGCCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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