Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27334 | 3' | -58.5 | NC_005857.1 | + | 3146 | 0.66 | 0.469393 |
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Target: 5'- uCUGugGCG-CUGCuGCAGgaGgGUa -3' miRNA: 3'- uGACugCGCuGACGuCGUCgaCgCGa -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 49071 | 0.66 | 0.459368 |
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Target: 5'- aGCUGGCGuCGGCUGUcaaAGCuucguuuguagAGUUGgGCUg -3' miRNA: 3'- -UGACUGC-GCUGACG---UCG-----------UCGACgCGA- -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 43240 | 0.66 | 0.456382 |
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Target: 5'- --aGACGCGAUUG-GGUacuugagccggaggAGCUGCGCa -3' miRNA: 3'- ugaCUGCGCUGACgUCG--------------UCGACGCGa -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 51458 | 0.66 | 0.449458 |
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Target: 5'- -aUGACGacuuccaccuCGACUGaGGCAGCUGCuGCc -3' miRNA: 3'- ugACUGC----------GCUGACgUCGUCGACG-CGa -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 16854 | 0.66 | 0.449458 |
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Target: 5'- uACUGAUGCaaaaGCUGguGacCGGCUGCaGCUg -3' miRNA: 3'- -UGACUGCGc---UGACguC--GUCGACG-CGA- -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 12191 | 0.67 | 0.401761 |
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Target: 5'- cGCUGACGCGuGCcGguGaCGGCU-CGCUg -3' miRNA: 3'- -UGACUGCGC-UGaCguC-GUCGAcGCGA- -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 48430 | 0.67 | 0.392614 |
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Target: 5'- uGCUGACGCGAUucugaUGCucccCAGCUG-GCa -3' miRNA: 3'- -UGACUGCGCUG-----ACGuc--GUCGACgCGa -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 15912 | 0.7 | 0.28584 |
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Target: 5'- cGCU--UGCGGCaggucgaUGCGGUGGCUGCGCUc -3' miRNA: 3'- -UGAcuGCGCUG-------ACGUCGUCGACGCGA- -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 26780 | 0.72 | 0.188607 |
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Target: 5'- uGCUcGACGCcGCacaGCAGCuGCUGCGCa -3' miRNA: 3'- -UGA-CUGCGcUGa--CGUCGuCGACGCGa -5' |
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| 27334 | 3' | -58.5 | NC_005857.1 | + | 3282 | 1.06 | 0.000536 |
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Target: 5'- cACUGACGCGACUGCAGCAGCUGCGCUu -3' miRNA: 3'- -UGACUGCGCUGACGUCGUCGACGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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