miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27335 5' -53.5 NC_005857.1 + 21330 0.66 0.819898
Target:  5'- gUCAGCGAUgcccCCUGGCuGGa-GAGGUCu -3'
miRNA:   3'- -AGUUGCUGuu--GGACUG-CCagCUCCGG- -5'
27335 5' -53.5 NC_005857.1 + 12203 0.66 0.819898
Target:  5'- cCggUGACGGCucgCUGgggguacgcGCGGUCGGGGgCg -3'
miRNA:   3'- aGuuGCUGUUG---GAC---------UGCCAGCUCCgG- -5'
27335 5' -53.5 NC_005857.1 + 34996 0.66 0.816166
Target:  5'- uUCuGCGACugugguauugauCCUGAaacacugacCGG-CGAGGCCg -3'
miRNA:   3'- -AGuUGCUGuu----------GGACU---------GCCaGCUCCGG- -5'
27335 5' -53.5 NC_005857.1 + 9703 0.66 0.791179
Target:  5'- gCAGCGGCGAUaaaaaaUGGCGGUgucgcagcagCGcAGGCUg -3'
miRNA:   3'- aGUUGCUGUUGg-----ACUGCCA----------GC-UCCGG- -5'
27335 5' -53.5 NC_005857.1 + 32937 0.67 0.739062
Target:  5'- cUCAAUGAUAagcGCCUGcaggGCGGUaggauaaucaaaaUGAGGCUc -3'
miRNA:   3'- -AGUUGCUGU---UGGAC----UGCCA-------------GCUCCGG- -5'
27335 5' -53.5 NC_005857.1 + 17718 0.68 0.729526
Target:  5'- cCGugGACGGCCUGGgugcaGGg-GAuGGCCa -3'
miRNA:   3'- aGUugCUGUUGGACUg----CCagCU-CCGG- -5'
27335 5' -53.5 NC_005857.1 + 22188 0.68 0.686299
Target:  5'- aUCAGCGcugaACAGUCUGACGGggCG-GGUCg -3'
miRNA:   3'- -AGUUGC----UGUUGGACUGCCa-GCuCCGG- -5'
27335 5' -53.5 NC_005857.1 + 5822 0.69 0.642228
Target:  5'- -aGACGGCAACaUGAaGGUCGAuuucuccaaagaGGCCu -3'
miRNA:   3'- agUUGCUGUUGgACUgCCAGCU------------CCGG- -5'
27335 5' -53.5 NC_005857.1 + 18641 0.69 0.620095
Target:  5'- uUCAACGacagucGCAugCUGACcGUCGcAGaGCCg -3'
miRNA:   3'- -AGUUGC------UGUugGACUGcCAGC-UC-CGG- -5'
27335 5' -53.5 NC_005857.1 + 31849 0.79 0.188312
Target:  5'- -gAACGACAGCCUGGCGGUucCGcaaguGGGUCa -3'
miRNA:   3'- agUUGCUGUUGGACUGCCA--GC-----UCCGG- -5'
27335 5' -53.5 NC_005857.1 + 4512 1.14 0.000774
Target:  5'- aUCAACGACAACCUGACGGUCGAGGCCg -3'
miRNA:   3'- -AGUUGCUGUUGGACUGCCAGCUCCGG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.